Development of three fusarium crown rot causal agents and systemic translocation of deoxynivalenol following stem base infection of soft wheat
Development of three fusarium crown rot causal agents and systemic translocation of deoxynivalenol following stem base infection of soft wheat
复制标题
DOI：
10.1111/ppa.12821
复制
发表时间：
2018-06-01
期刊：
影响因子：
2.7
通讯作者：
Covarelli, L.
中科院分区：
文献类型：
--
作者：
Beccari, G.;Prodi, A.;Covarelli, L.
假禾谷镰刀菌、黄镰刀菌和禾谷镰刀菌是最重要的镰刀菌冠腐病 (FCR) 病原。它们具有生物合成脱氧雪腐镰刀菌烯醇 (DON) 的共同能力。为了阐明这三个物种的行为，进行了一项比较研究，以调查软质小麦茎基部接种后的症状进展、真菌系统生长和 DON 易位。 FCR症状主要集中在接种区域，所有接种物种均延伸至第二个节点。只有最具攻击性的菌株才会引起直至第三节的症状。实时定量 PCR 显示，所有测试物种的真菌定殖均达到了第三节，但在接种最具攻击性的菌株后，很少有植物在第三节以上表现出定殖。在无症状的组织中检测到真菌生长，但这三个物种中没有一个能够定植到头部组织。然而，即使在头部没有检测到病原体，在接种最具攻击性菌株的植物的头部组织中也检测到了 DON。这些结果表明，在相同的实验条件下，F.pseudograminearum、F.culmorum 和 F.graminearum 在茎基部感染后遵循相似的症状进展、真菌定植和 DON 易位模式。症状程度、真菌定植和霉菌毒素分布的差异主要归因于菌株的攻击性。这些发现提供了三种最重要的 FCR 偶然病原体感染小麦茎基部后事件的比较信息。
Fusarium pseudograminearum, F.culmorum and F.graminearum are the most important fusarium crown rot (FCR) causal agents. They have the common ability to biosynthesize deoxynivalenol (DON). To elucidate the behaviour of each of the three species, a comparative study was carried out to investigate symptom progression, fungal systemic growth and translocation of DON following stem base inoculation of soft wheat. FCR symptoms were mainly localized in the inoculated area, which extended up to the second node for all inoculated species. Only the most aggressive strains caused symptoms up to the third node. Real-time quantitative PCR showed that fungal colonization reached the third node for all the tested species, but a low percentage of plants showed colonization above the third node following inoculation with the most aggressive strains. Fungal growth was detected in symptomless tissues but none of the three species was able to colonize as far as the head tissues. However, even if the pathogens were not detected in the heads, DON was detected in head tissues of the plants inoculated with the most aggressive strains. These results demonstrate that F.pseudograminearum, F.culmorum and F.graminearum, under the same experimental conditions, follow a similar pattern of symptom progression, fungal colonization and DON translocation after stem base infection. Differences in the extent of symptoms, fungal colonization and mycotoxin distribution were mainly attributable to strain aggressiveness. These findings provide comparative information on the events following infection of the stem base of wheat by three of the most important FCR casual agents.