Statistics of DNA Capture by a Solid-State Nanopore
Statistics of DNA Capture by a Solid-State Nanopore
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DOI：
10.1103/physrevlett.110.028102
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发表时间：
2013-01-07
影响因子：
8.6
通讯作者：
Stein, Derek
中科院分区：
文献类型：
--
作者：
Mihovilovic, Mirna;Hagerty, Nicholas;Stein, Derek
固态纳米孔可以捕获DNA分子并将其以折叠构型拉过。所得离子电流信号指示DNA在沿着其长度的何处被捕获。使用8 nm宽的纳米孔的统计研究揭示了有利于捕获分子末端附近的强烈偏好。一个理论模型表明，偏置的结果，而不是一个搜索的聚合物的构型熵的积极有利的配置。我们还量化的波动和长度依赖的速度，同时易位聚合物片段从我们的研究折叠的DNA配置。DOI：10.1103/PhysRevLett.110.028102
A solid-state nanopore can electrophoretically capture a DNA molecule and pull it through in a folded configuration. The resulting ionic current signal indicates where along its length the DNA was captured. A statistical study using an 8-nm-wide nanopore reveals a strong bias favoring the capture of molecules near their ends. A theoretical model shows that bias to be a consequence of configurational entropy rather than a search by the polymer for an energetically favorable configuration. We also quantified the fluctuations and length dependence of the speed of simultaneously translocating polymer segments from our study of folded DNA configurations. DOI: 10.1103/PhysRevLett.110.028102