Eda/Edar signaling guides fin ray formation with preceding osteoblast differentiation, as revealed by analyses of the medaka all-fin less mutant afl
Eda/Edar signaling guides fin ray formation with preceding osteoblast differentiation, as revealed by analyses of the medaka all-fin less mutant afl
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DOI：
10.1002/dvdy.24120
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发表时间：
2014-06-01
影响因子：
2.5
通讯作者：
Kudo, Akira
中科院分区：
文献类型：
--
作者：
Iida, Yuuki;Hibiya, Kenta;Kudo, Akira
背景：外胚层发育异常蛋白(EDA)信号对几种外胚层附属物的形态发生是必不可少的。结果：在这里，我们报告了一个青竹突变体，All-Fin Less(AFL)，它的eda基因有一个无义突变。成年AFL鱼的皮肤骨架显示出各种异常，如鳍条短而扭曲，鳞片和牙齿缺失或形状异常，以及头骨变形。对AFL幼虫尾部发育的鳍射线进行了观察，发现虽然鳍射线的初始形成过程正常，但鳍射线并未伸长。此外，EDA表达缺失，EDA受体基因EDAR的表达模式与野生型不同。体内成像显示，在EDAR启动子和Osterix启动子控制下的DsRed控制下的双转基因青竹表达增强型绿色荧光蛋白，EDAR先于Osterix表达，并且表达EDAR的细胞沿鳍线延伸方向迁移，表明Eda/Edar信号事件先于成骨细胞分化。结论：我们的发现提供了证据，即伴随着EDA与EDAR结合的EDA信号对于由细胞迁移引导的鳍线的形成是必不可少的。《发展动力学243：765-777》，2014。(C)2014年威利期刊公司。
Background: Ectodysplasin (Eda) signaling is essential for the morphogenesis of several ectodermal appendages. Results: Here, we report a medaka mutant, all-fin less (afl), which has a nonsense mutation in its eda gene. The adult afl fish displayed various abnormalities of its dermal skeleton, such as short and twisted fin rays, missing and abnormally shaped scales and teeth, and skull deformation. Focusing on the developing fin rays in the caudal region of afl larvae, we found that the fin rays did not elongate; although the initial formation of fin rays proceeded normally. Additionally, eda expression was lost, and the expression pattern of edar, the gene for the receptor of Eda, was different from wild-type one. In vivo imaging of the double-transgenic medaka expressing enhanced green fluorescent protein under control of the edar promoter and DsRed under control of the osterix promoter revealed that edar expression preceded that of osterix and that the edar-expressing cells migrated in the direction of fin ray elongation, indicating that the Eda/Edar signaling event precedes osteoblast differentiation. Conclusions: Our findings provide evidence that Eda signaling accompanied with the binding of Eda to Edar are essential for fin ray formation guided by cell migration. Developmental Dynamics 243:765-777, 2014. (c) 2014 Wiley Periodicals, Inc.