Acetate Production from Cafeteria Wastes and Corn Stover Using a Thermophilic Anaerobic Consortium: A Prelude Study for the Use of Acetate for the Production of Value-Added Products
Acetate Production from Cafeteria Wastes and Corn Stover Using a Thermophilic Anaerobic Consortium: A Prelude Study for the Use of Acetate for the Production of Value-Added Products
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DOI：
10.3390/microorganisms8030353
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发表时间：
2020-03-01
期刊：
影响因子：
4.5
通讯作者：
Sani, Rajesh Kumar
中科院分区：
文献类型：
--
作者：
David, Aditi;Tripathi, Abhilash Kumar;Sani, Rajesh Kumar
利用低成本废物进行高效和可持续的生化生产具有重要的工业和生态意义。固体有机废物（SOWs）是一种价格低廉、可利用性丰富的资源，它们的生物转化为挥发性脂肪酸，特别是乙酸，有助于减轻工业微生物生化生产对纯糖的需求。如果环境因素得到优化，利用这些废物的有机碳从SOW中生产乙酸是一种有效的固体废物减少策略。本研究筛选并优化了两种sows -自助餐厅废弃物和玉米秸秆在嗜热产醋酸菌群生产醋酸盐的影响因素（物理和化学）。筛选实验表明，温度、溴乙烷磺酸盐和摇摇对乙酸酯产量有显著影响。采用响应面法进一步优化温度、培养基pH、C:N比。在温度、pH、C:N分别为60℃、6℃、25℃时，乙酸浓度达到8061 mg L-1的最大值，提高了200%，乙酸占代谢产物的85%以上。本研究还证明了利用（从sow中获得的）富含乙酸的发酵液作为底物生长工业相关酵母的可行性，该酵母可以将乙酸转化为高价值的生化物质。
Efficient and sustainable biochemical production using low-cost waste assumes considerable industrial and ecological importance. Solid organic wastes (SOWs) are inexpensive, abundantly available resources and their bioconversion to volatile fatty acids, especially acetate, aids in relieving the requirements of pure sugars for microbial biochemical productions in industries. Acetate production from SOW that utilizes the organic carbon of these wastes is used as an efficient solid waste reduction strategy if the environmental factors are optimized. This study screens and optimizes influential factors (physical and chemical) for acetate production by a thermophilic acetogenic consortium using two SOWs-cafeteria wastes and corn stover. The screening experiment revealed significant effects of temperature, bromoethane sulfonate, and shaking on acetate production. Temperature, medium pH, and C:N ratio were further optimized using statistical optimization with response surface methodology. The maximum acetate concentration of 8061 mg L-1 ( >200% improvement) was achieved at temperature, pH, and C:N ratio of 60 degrees C, 6, 25, respectively, and acetate accounted for more than 85% of metabolites. This study also demonstrated the feasibility of using acetate-rich fermentate (obtained from SOWs) as a substrate for the growth of industrially relevant yeast Yarrowia lipolytica, which can convert acetate into higher-value biochemicals.