Simultaneous analysis of artemisinin and flavonoids of several extracts of Artemisia annua L. obtained from a commercial sample and a selected cultivar
Simultaneous analysis of artemisinin and flavonoids of several extracts of Artemisia annua L. obtained from a commercial sample and a selected cultivar
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DOI：
10.1016/j.phymed.2006.01.008
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发表时间：
2006-07-01
期刊：
影响因子：
7.9
通讯作者：
Vincieri, F. F.
中科院分区：
文献类型：
--
作者：
Bilia, A. R.;de Malgalhaes, P. Melillo;Vincieri, F. F.
[医]青蒿青蒿是一种很有前途的强效抗疟中药。这种活性归因于其成分青蒿素，青蒿素是一种萜烯内酯，对耐药疟原虫物种非常有效，毒性低。研究表明，A.青蒿素能促进和增强青蒿素与氯化血红素的反应。这些数据是在良好的协议与以前的调查在体外增强抗疟活性的青蒿素由这样的flavonoids.As的结果，鉴于可能使用的植物复合物，而不是纯青蒿素，HPLC/DAD/MS方法提出了同时检测和定量的黄酮类化合物和青蒿素。从两种不同的草药，商业样品和一个选定的品种，获得不同的提取物进行了分析，以确定溶剂提供青蒿素和黄酮类化合物的最佳产量。定性和定量的结果，使用HPLC方法进行了描述，这将是有用的开发高效的草药制剂。(c)2006年Elsevier GmbH。All rights reserved.
Arteirtisia annua L. (Qinghao) is a promising and potent antimalarial herbal drug. This activity has been ascribed to its component artemisinin, a sesquiterpene lactone that is very effective against drug-resistant Plasmodium species with a low toxicity. Our studies indicate that several flavonoids of A. annua can promote and enhance the reaction of artemisinin with hemin. These data are in good agreement with previous investigations on the in vitro potentiation of antimalarial activity of artemisinin by such flavonoids.As a consequence, in view of a possible use of the phytocomplex rather than pure artemisinin, an HPLC/DAD/MS method is proposed for the simultaneous detection and quantification of both flavonoids and artemisinin. Different extracts, obtained from two different herbal drugs, a commercial sample and a selected cultivar, were analyzed in order to determine which solvents provide the best yields of both artemisinin and flavonoids. Qualitative and quantitative results obtained using an HPLC method are described, which will be useful for developing highly effective herbal drug preparations. (c) 2006 Elsevier GmbH. All rights reserved.