Design and fabrication of flexible DNA polymer cocoons to encapsulate live cells
Design and fabrication of flexible DNA polymer cocoons to encapsulate live cells
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DOI：
10.1038/s41467-019-10845-2
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发表时间：
2019-07-03
影响因子：
16.6
通讯作者：
Li, Genxi
中科院分区：
文献类型：
--
作者：
Gao, Tao;Chen, Tianshu;Li, Genxi
将指定的活细胞封装到生物和机械可调聚合物层中的能力非常迫切。在此，提出了一种编织功能性 DNA 聚合物茧的方法作为封装方法。通过开发原位 DNA 定向聚合 (isDOP)，我们展示了一种将 DNA 聚合物移植到活细胞上的局部、可编程和生物相容性封装方法。在两个相互辅助的酶反应的进一步引导下，嫁接的 DNA 聚合物在细胞表面组装成 DNA 聚合物茧。因此，实现了细菌、酵母、哺乳动物细胞的包被。这种方法的功能可能为设计细胞表面提供重要机会，并能够对封装的细胞进行精确操作，例如编码、处理和分选，适用于许多生物医学应用。
The capability to encapsulate designated live cells into a biologically and mechanically tunable polymer layer is in high demand. Here, an approach to weave functional DNA polymer cocoons has been proposed as an encapsulation method. By developing in situ DNA-oriented polymerization (isDOP), we demonstrate a localized, programmable, and biocompatible encapsulation approach to graft DNA polymers onto live cells. Further guided by two mutually aided enzymatic reactions, the grafted DNA polymers are assembled into DNA polymer cocoons at the cell surface. Therefore, the coating of bacteria, yeast, and mammalian cells has been achieved. The capabilities of this approach may offer significant opportunities to engineer cell surfaces and enable the precise manipulation of the encapsulated cells, such as encoding, handling, and sorting, for many biomedical applications.