Effects of CO2 lasers on dental pulp biology in rats.
Effects of CO2 lasers on dental pulp biology in rats.
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CO2 激光对大鼠牙髓生物学的影响。
DOI：
10.1089/pho.2015.3997
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发表时间：
2016
影响因子：
--
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Ser-Od;T.;Yasumoto;M.;Al-Wahabi;A.;Nakajima;K.;Murakami;S.;Matsuzaka;K.;Inoue;T.
目的：应用逆转录聚合酶链反应（RT-PCR）和免疫组织化学方法，研究CO2激光照射对大鼠牙髓细胞增殖、分化及潜在自我修复能力的影响。用波长为10，600 nm的CO2激光在相同参数下（2 W CO2激光，能量4J，能量密度203.84 J/cm 2，照射8.8 s）通过咬合面的牙本质照射上颌第一磨牙。在激光照射后即刻、1、3或5天拔除磨牙。采用热休克蛋白70（Hsp 70）、三磷酸腺苷（ATP）结合盒转运蛋白G2（ABCG 2）、牙本质涎磷蛋白（DSPP）和牙本质基质蛋白1（DMP 1）特异性引物进行RT-PCR分析，并采用增殖细胞核抗原（PCNA）、ABCG 2、CD 34和CD 44特异性抗体进行免疫组织化学分析。RT-PCR分析显示，Hsp 70 mRNA在即刻组表达最高，ABCG 2 mRNA在第1天表达最高。激光照射组DSPP和DMP 1 mRNA表达逐渐增加，第5天达高峰，但各组间DMP 1表达无显著差异。免疫组化，PCNA阳性细胞在激光照射后的所有时间观察到，但是，他们是最明显的第3天。CD 44阳性细胞在第1、3天较强，ABCG 2阳性细胞在第3天最明显。结论：CO2激光照射后牙髓组织发生退变，并由新生的成牙本质细胞样细胞修复。
Objective:The purpose of this study was to investigate the effects of CO2lasers on the proliferation and differentiation of dental pulp cells, and their latent self-recovery in connection with their stemness using reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry.Materials and methods:The first molars from male Sprague–Dawley rats, each weighing ∼150–200 g, were used for this study. The upper first molars were irradiated with a 10,600 nm wavelength CO2laser under identical parameters (2 W CO2laser, energy 4J, energy density 203.84 J/cm2for 8.8 sec) through the dentin of the occlusal surface. The molars were extracted immediately, or at 1, 3 or 5 days after the laser irradiation. RT-PCR analysis using primers specific for heat shock protein 70 (Hsp70), adenosine triphosphate (ATP)-binding cassette transporter G2 (ABCG2), dentin sialophosphoprotein (DSPP), and dentin matrix protein 1 (DMP1), and immunohistochemistry using antibodies specific for proliferating cell nuclear antigen (PCNA), ABCG2, CD34, and CD44 were performed.Results:RT-PCR analysis revealed that Hsp70 mRNA expression in the immediate group and ABCG2 mRNA expression at day 1 were the highest. DSPP and DMP1 mRNA expression in the laser-irradiated groups increased gradually, reaching its peak on the 5th day of the experiment, although no significant difference found among groups with regard to DMP1 expression. Immunohistochemically, PCNA-positive cells were observed at all times after the laser irradiation; however, they were most evident on day 3. CD44-positive cells were observed strongly on day 1 and day 3, while ABCG2-positive cells were the most evident on day 3.Conclusions:These results demonstrate that CO2laser irradiation induces degeneration in the pulp tissue, which is then repaired by newly formed odontoblast-like cells.