Bronchoalveolar lavage fluid analysis in individuals with chronic hepatitis C
Bronchoalveolar lavage fluid analysis in individuals with chronic hepatitis C
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DOI：
10.1002/jmv.2108
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发表时间：
2002-01-01
影响因子：
12.7
通讯作者：
Dökmeci, A
中科院分区：
文献类型：
--
作者：
Idilman, R;Çetinkaya, H;Dökmeci, A
与丙型肝炎病毒感染存在关联的一些疾病。这些疾病包括原发性混合冷球蛋白血症、膜增生性肾小球肾炎和特发性肺纤维化。本研究旨在调查慢性丙型肝炎患者支气管肺泡灌洗液的细胞含量和淋巴细胞亚群，并将结果与对照组进行比较。研究对象为18例慢性丙型肝炎患者（男/女，6/12）和14例健康志愿者（男/女，6/8）。分别取支气管肺泡灌洗液；测定支气管肺泡灌洗液淋巴细胞亚型及HCV-RNA的存在。抗- hcv阳性受试者血清HCV-RNA均呈阳性。1例（5.6%）支气管肺泡灌洗HCV-RNA阳性。慢性丙型肝炎患者支气管肺泡灌洗液的总细胞和中性粒细胞计数明显高于对照组(5,799.6 +/- 957.4 × 10(3)/ml vs. 1,835.7 +/- 447.8 × 10(3)/ml, P = 0.001；1175 .8 + / - 634.7 x 10(3) /毫升和53.1 + / - 28.1 x 10(3) /毫升,P = 0.029)。相比之下，淋巴细胞、巨噬细胞和嗜酸性粒细胞计数没有差异。两组间支气管肺泡灌洗液中单个T细胞亚群或B细胞数量的百分比、中位数或范围均无差异。结论是，丙型肝炎病毒感染可能与支气管肺泡灌洗液中多形核中性粒细胞数量增加所表现的隐性肺部炎症反应有关，这一发现可能有助于在少数慢性丙型肝炎病例中导致特发性肺纤维化(C)。
A number of disorders for which an association with hepatitis C virus infection exist. These disorders include essential mixed cryoglobulinemia, membranoproliferative glomerulonephritis, and idiopathic pulmonary fibrosis. This study was initiated to investigate the cellular content and lymphocyte subpopulations of bronchoalveolar lavage fluid obtained from individuals with chronic hepatitis C and to compare the results to those of controls. Eighteen patients with chronic hepatitis C (male/female, 6/12) and 14 healthy volunteers (male/female, 6/8), were studied. Bronchoalveolar lavage fluid was obtained from each; and the lymphocyte subtypes and the presence of HCV-RNA in the bronchoalveolar lavage fluid were determined. All anti-HCV positive subjects were HCV-RNA positive in serum. One (5.6%) had a HCV-RNA positive bronchoalveolar lavage. The total cell and neutrophil counts of the bronchoalveolar lavage fluid were significantly greater in patients with chronic hepatitis C as compared to controls (5,799.6 +/- 957.4 x 10(3)/ml vs. 1,835.7 +/- 447.8 x 10(3)/ml, P = 0.001; 1,175.8 +/- 634.7 x 10(3)/ml vs. 53.1 +/- 28.1 x 10(3)/ml, P = 0.029). In contrast, the lymphocyte, macrophage and eosinophil counts did not differ. No difference in the percentage, median or range of individual T cell subsets or B cell numbers in the bronchoalveolar lavage fluid existed between the groups. It is concluded that hepatitis C virus infection may be associated with an occult pulmonary inflammatory reaction manifested by an increased number of polymorphonuclear neutrophils in bronchoalveolar lavage fluid This finding may contribute to the process that leads to idiopathic pulmonary fibrosis seen in a minority of cases of chronic hepatitis C. (C) 2002 Wiley-Liss, Inc.