Atypical centrioles are present in Tribolium sperm.
Atypical centrioles are present in Tribolium sperm.
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DOI：
10.1098/rsob.160334
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发表时间：
2017-03
期刊：
影响因子：
5.8
通讯作者：
Avidor-Reiss T
中科院分区：
文献类型：
--
作者：
Fishman EL;Jo K;Ha A;Royfman R;Zinn A;Krishnamurthy M;Avidor-Reiss T
典型的中心粒是由微管组成的，微管排列成九重对称。大多数动物体细胞具有两个中心粒，用于正常的细胞分裂和功能。这些中心粒起源于受精卵，但由于卵母细胞不提供任何中心粒，令人惊讶的是，许多动物的受精卵被认为只从精子继承了一个中心粒。最近，在果蝇精子中，我们发现了第二个中心粒结构，近端中心粒样结构（PCL），它在受精卵中起作用。其他昆虫的精子是否具有第二中心粒结构尚不清楚。在这里，我们的特点精子发生在红粉甲虫，赤拟谷盗。电子显微镜显示拟谷盗的轴丝顶端有一个以微管为基础的中心粒，其结构类似于PCL，缺少微管，位于细胞核的胞质内陷处。对中心粒/PCL蛋白的直系同源物Ana 1的免疫染色也识别出精子发生过程中细胞核附近的两个中心粒：一个是基于微管的轴丝尖端，表明它是中心粒;另一个是更近端的，出现在精子发生早期，表明它是PCL。总之，这些发现表明拟谷盗精子具有一个基于微管的中心粒和一个缺乏微管的中心粒。
Typical centrioles are made of microtubules organized in ninefold symmetry. Most animal somatic cells have two centrioles for normal cell division and function. These centrioles originate from the zygote, but because the oocyte does not provide any centrioles, it is surprising that the zygotes of many animals are thought to inherit only one centriole from the sperm. Recently, in the sperm of Drosophila melanogaster, we discovered a second centriolar structure, the proximal centriole-like structure (PCL), which functions in the zygote. Whether the sperm of other insects has a second centriolar structure is unknown. Here, we characterized spermiogenesis in the red flour beetle, Tribolium castaneum. Electron microscopy suggests that Tribolium has one microtubule-based centriole at the tip of the axoneme and a structure similar to the PCL, which lacks microtubules and lies in a cytoplasmic invagination of the nucleus. Immunostaining against the orthologue of the centriole/PCL protein, Ana1, also recognizes two centrioles near the nucleus during spermiogenesis: one that is microtubule-based at the tip of the axoneme, suggesting it is the centriole; and another that is more proximal and appears during early spermiogenesis, suggesting it is the PCL. Together, these findings suggest that Tribolium sperm has one microtubule-based centriole and one microtubule-lacking centriole.