Validation and efficacy of transgenerational mass marking of otoliths in viviparous fish larvae.
Validation and efficacy of transgenerational mass marking of otoliths in viviparous fish larvae.
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DOI：
10.1111/j.1095-8649.2010.02681.x
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发表时间：
2010-07
影响因子：
2
通讯作者：
M. Kuroki;R. Buckley;L. Leclair;L. Hauser
中科院分区：
文献类型：
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作者：
M. Kuroki;R. Buckley;L. Leclair;L. Hauser
通过对妊娠雌性鱼肌肉注射氯化锶(SrCl2)元素和发育中仔鱼耳石中锶的检测，验证了胎生仔鱼体内跨代质量标记的有效性。注射SrCl2的褐岩鱼幼鱼的所有耳石都在耳石边缘附近表现出丰富的锶/钙比值，其特征似乎不受仔鱼在卵巢内的前、中心和后部位置的影响。本研究的结果表明，对于大量极小胎生鱼类幼体的标记，跨代标记是一种高度可靠的技术。
Transgenerational mass marking of viviparous fish larvae in vivo was validated by intra-muscular injection of elemental strontium chloride (SrCl(2)) in gestating females and detection of the Sr in the otoliths of developing larvae. All otoliths of brown rockfish Sebastes auriculatus larvae produced from SrCl(2)-injected females showed enriched Sr:Ca ratios near the otolith edges, and the signatures did not appear to be affected by the anterior, centre and posterior positions of larvae within the ovary. Results from the present study indicate that transgenerational marking is a highly reliable technique for marking large numbers of extremely small viviparous fish larvae.