An introduction to the wound healing assay using live-cell microscopy
An introduction to the wound healing assay using live-cell microscopy
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DOI：
10.4161/cam.36224
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发表时间：
2014-09-01
影响因子：
3.2
通讯作者：
Colarusso, Pina
中科院分区：
文献类型：
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作者：
Jonkman, James E. N.;Cathcart, Judith A.;Colarusso, Pina
伤口愈合测定在一系列学科中用于研究细胞群体的协调运动。在这篇技术综述中，我们描述了通过光学显微镜监测的伤口愈合测定的工作流程。虽然该检测方法简单明了，但由于其应用缺乏标准化，因此很难在研究人员之间比较结果和重现实验。我们建议一致性的一般指导原则，包括：（1）样品制备，包括创建差距，（2）显微镜设备要求，（3）图像采集，以及（4）使用图像分析测量差距大小及其随时间的闭合率。我们还描述了具体的研究问题，如播种密度和基质涂层的参数。所有这些参数必须在给定的一组实验中仔细控制，以获得准确和可重复的结果。
The wound healing assay is used in a range of disciplines to study the coordinated movement of a cell population. In this technical review, we describe the workflow of the wound healing assay as monitored by optical microscopy. Although the assay is straightforward, a lack of standardization in its application makes it difficult to compare results and reproduce experiments among researchers. We recommend general guidelines for consistency, including: (1) sample preparation including the creation of the gap, (2) microscope equipment requirements, (3) image acquisition, and (4) the use of image analysis to measure the gap size and its rate of closure over time. We also describe parameters that are specific to the particular research question, such as seeding density and matrix coatings. All of these parameters must be carefully controlled within a given set of experiments in order to achieve accurate and reproducible results.