Characterization of Oil Palm Acyl-CoA-Binding Proteins and Correlation of Their Gene Expression with Oil Synthesis
Characterization of Oil Palm Acyl-CoA-Binding Proteins and Correlation of Their Gene Expression with Oil Synthesis
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DOI：
10.1093/pcp/pcz237
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发表时间：
2020-04-01
影响因子：
4.9
通讯作者：
Low, Eng-Ti Leslie
中科院分区：
文献类型：
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作者：
Amiruddin, Nadzirah;Chan, Pek-Lan;Low, Eng-Ti Leslie
酰基辅酶a结合蛋白（acbp）在真核细胞中参与酰基辅酶a酯的结合和运输。acbp含有一个保守的酰基辅酶a结合域。它们的各种功能已经在模式植物拟南芥中得到了表征，在较小程度上，在水稻中也得到了表征。本研究对世界上最重要的油料作物Elaeis guineensis （oil palm）进行了acbp的全基因组检测和表达分析。根据推导出的氨基酸结构域结构，鉴定出7个豚鼠acbp，并将其分为4类。系统发育分析表明，该科与其他高等植物保持一致。所有7种egacbp均在大多数组织中表达，其差异表达提示其在特定组织中具有不同的功能。例如，EgAC8P3在花序和茎中表达量高，而EgACBP1在叶片中表达量高。由于几内亚赤霉素作为油料作物的重要性，egacbp的表达在果实发育过程中被专门检测。EgACBP3在中果皮发育过程中高表达，而EgACBP1在油脂快速合成过程中表达增强。在胚乳中，EgACBP1和EgACBP3在种子发育过程中表达增加。这些结果为进一步研究egacbp在各种组织中的生物学功能，特别是其在油脂合成中的作用提供了重要信息。
Acyl-CoA-binding proteins (ACBPs) are involved in binding and trafficking acyl-CoA esters in eukaryotic cells. ACBPs contain a well-conserved acyl-CoA-binding domain. Their various functions have been characterized in the model plant Arabidopsis and, to a lesser extent, in rice. In this study, genome-wide detection and expression analysis of ACBPs were performed on Elaeis guineensis (oil palm), the most important oil crop in the world. Seven E guineensis ACBPs were identified and classified into four groups according to their deduced amino acid domain organization. Phylogenetic analysis showed conservation of this family with other higher plants. All seven EgACBPs were expressed in most tissues while their differential expression suggests various functions in specific tissues. For example, EgAC8P3 had high expression in inflorescences and stalks while EgACBP1 showed strong expression in leaves. Because of the importance of F. guineensis as an oil crop, expression of EgACBPs was specifically examined during fruit development. EgACBP3 showed high expression throughout mesocarp development, while EgACBP1 had enhanced expression during rapid oil synthesis. In endosperm, both EgACBP1 and EgACBP3 exhibited increased expression during seed development. These results provide important information for further investigations on the biological functions of EgACBPs in various tissues and, in particular, their roles in oil synthesis.