The nematode Panagrellus redivivus is susceptible to killing by human pathogens at 37 °C
The nematode Panagrellus redivivus is susceptible to killing by human pathogens at 37 °C
复制标题
DOI：
10.1016/j.femsle.2005.06.046
复制
发表时间：
2005-09-01
影响因子：
2.1
通讯作者：
Titball, RW
中科院分区：
文献类型：
--
作者：
Laws, TR;Smith, SA;Titball, RW
秀丽隐杆线虫已被用作研究哺乳动物致病细菌的宿主。然而，该模型的一个重要限制是，秀丽线虫在37摄氏度下不能存活。我们报告说，生殖分布线虫Panagrellus redivivus生存在37摄氏度，并保持其生命周期的温度高达31.5摄氏度，包括。线虫病原体铜绿假单胞菌、肠道沙门氏菌、金黄色葡萄球菌，而非假结核耶尔森氏菌，减少了P.redivus的寿命。在测试的四种多食伯克霍尔德氏菌菌株中，一种在两种温度下都降低了P.redivus的寿命，一种在两种温度下都是无毒的，两种菌株仅在37 ℃下降低了P.redivus的寿命。进一步研究了这些菌株之一在37 ℃下杀死P.redivivus而不是在25 ℃下杀死P.redivus的机制。杀死所需的活菌，不涉及组织的细菌入侵，不太可能是由于扩散性细菌毒素，也与蠕虫肠道内活菌数量增加无关。我们认为多噬红球藻可能通过类似于B的温度调节机制杀死红球藻。类鼻疽致死C.优雅(c)2005年，欧洲微生物学会联合会。Elsevier B.V.出版，保留所有权利。
Caenorhabditis elegans has been used as a host for the study of bacteria that cause disease in mammals. However, a significant limitation of the model is that C elegans is not viable at 37 degrees C. We report that the gonochoristic nematode Panagrellus redivivus survives at 37 degrees C and maintains its life cycle at temperatures up to and including 31.5 degrees C. The C elegans pathogens Pseudomonas aeruginosa, Salmonella enterica, Staphylococcus aureus, but not Yersinia pseudo tuberculosis, reduced P. redivivus lifespan. Of four strains of Burkholderia multivorans tested, one reduced P. redivivus lifespan at both temperatures, one was avirulent at both temperatures and two strains reduced P. redivivus lifespan only at 37 degrees C. The mechanism by which one of these strains killed P. redivivus at 37 degrees C, but not at 25 degrees C, was investigated further. Killing required viable bacteria, did not involve bacterial invasion of tissues, is unlikely to be due to a diffusible, bacterial toxin and was not associated with increased numbers of live bacteria within the intestine of the worm. We believe R multivorans may kill P. redivivus by a temperature-regulated mechanism similar to B. pseudomallei killing of C. elegans. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.