Physiology and gene expression of the rice landrace Horkuch under salt stress
Physiology and gene expression of the rice landrace Horkuch under salt stress
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DOI：
10.1071/fp10198
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发表时间：
2011-01-01
影响因子：
3
通讯作者：
Seraj, Zeba I.
中科院分区：
文献类型：
--
作者：
Lisa, Laisa A.;Elias, Sabrina M.;Seraj, Zeba I.
在不断变化的气候条件下，良好的耐盐育种捐赠者是粮食安全的先决条件。霍尔库赫，农民喜闻乐见的耐盐水稻(Oryza sativa L.)来自孟加拉国西南沿海的水稻品种在盐胁迫下一直到成熟，以及现代品种(BRRI Dhan41)、敏感对照(BRRI Dhan29)和Pokkali，这是水稻的耐盐性基准。霍尔库赫地上部生物量减少率低，旗叶Na/K比低，产量减少率低，Na在老叶中分配良好，旗叶中保持较高的钙、镁含量。为了从分子水平上了解盐胁迫的生理机制，利用基因芯片技术对盐胁迫相关基因的表达进行了研究。将盐胁迫下的霍尔库赫幼苗的cDNA与主要从拟南芥数据库序列中合成的cDNA探针杂交。海因。上调的基因包括转录因子、信号转导、代谢酶、活性氧清除剂、渗透保护剂和一些盐诱导的特异转录本。光合作用相关基因以及ROS清除剂表达的增加表明，这可能是Horkuch产量表现较好的原因。因此，这些数据表明，在耐盐性育种计划中，霍尔库赫是Pokkali的潜在供体替代品。
Good donors in breeding for salt tolerance are a prerequisite for food security under changing climatic conditions. Horkuch, a farmer-popular salt tolerant rice (Oryza sativa L.) variety from the south-west coast of Bangladesh was characterised up to maturity under NaCl stress, together with a modern variety (BRRI dhan41), a sensitive control (BRRI dhan29) and Pokkali, the salt-tolerant benchmark for rice. Horkuch had low reduction in shoot biomass, a low Na : K ratio in flag leaves, a low percent reduction in yield and good partitioning of Na in the older leaves, and maintained high levels of Ca and Mg in the flag leaves. In order to understand the physiology at the molecular level, the expression of salt-responsive genes was investigated using microarray analysis. Salt-stressed cDNA of Horkuch seedlings were hybridised with cDNA probes synthesised mainly from database sequences of Arabidopsis thaliana (L.) Heynh. The upregulated genes included transcription factors, signal transducers, metabolic enzymes, reactive oxygen species (ROS) scavengers, osmoprotectants and some specific salt-induced transcripts. An increase in expression of photosynthesis-related genes as well ROS scavengers suggested that this could be the reason for the better yield performance of Horkuch. The data therefore indicate Horkuch as a potential donor alternative to Pokkali in breeding programs for salt tolerance.