Accuracy of aqueous humor flow determination by fluorophotometry.
Accuracy of aqueous humor flow determination by fluorophotometry.
复制标题
通过荧光光度法测定房水流量的准确性。
DOI：
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发表时间：
1978
影响因子：
4.4
通讯作者：
H. MacLellan
中科院分区：
文献类型：
--
作者：
J. Pederson;D. Gaasterland;H. MacLellan
在荧光素的角膜离子电渗后，用谷胱甘肽-碳酸氢盐-林格氏溶液以已知速率灌注新鲜摘除的恒河猴眼睛。每15分钟用荧光光度计测量总荧光素质量和水性荧光素浓度，持续6至8小时。通过Jones和Maurice的第二方法从荧光光度计数据计算水性流量。然后将该流量与已知的灌注速率进行比较。总荧光衰减的曲线并不精确地平行于水性荧光衰减的曲线。这影响了总荧光：水性荧光的比率的值。最准确和一致的流量值是用来自水性荧光衰减曲线的衰减常数与外推回零时间的总：水性荧光的比率组合获得的。在这两种条件下，五个实验中的计算流量为5.4% +/- 3.3（+/- S.D.）低于已知流量。已知流量和计算流量之间的真实平均百分比差异的99%置信区间为-12%至+1%。观察到角膜荧光素浓度对测量的水性荧光的小影响。
After corneal iontophoresis of fluorescein, freshly enucleated rhesus monkey eyes were perfused at a known rate with glutathione-bicarbonate-Ringer's solution. Total fluorescein mass and aqueous fluorescein concentration were measured with a fluorophotometer every 15 min for 6 to 8 hr. The aqueous flow was calculated from the fluorophotometry data by the second method of Jones and Maurice. This flow was then compared to the known perfusion rate. The curve for total fluorescence decay was not precisely parallel to the curve for aqueous fluorescence decay. This affected the value of the ratio of total:aqueous fluorescence. The most accurate and consistent flow values were obtained with the decay constant from the aqueous fluorescence decay curve combined with the ratio of total:aqueous fluorescence extrapolated back to zero time. Under these two conditions, the calculated flow in five experiments was 5.4% +/- 3.3 (+/- S.D.) lower than the known flow. The 99% confidence interval of the true mean percent difference between the known and calculated flows is--12% to +1%. A small effect of corneal fluorescein concentration on measured aqueous fluorescence was observed.