Effects of NO/cGMP Signaling on Behavioral Changes in Subordinate Male Crickets, Gryllus bimaculatus
Effects of NO/cGMP Signaling on Behavioral Changes in Subordinate Male Crickets, Gryllus bimaculatus
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DOI：
10.2108/zsj.24.860
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发表时间：
2007-09
期刊：
影响因子：
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通讯作者：
M. Iwasaki;H. Nishino;A. Delago;H. Aonuma
中科院分区：
文献类型：
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作者：
M. Iwasaki;H. Nishino;A. Delago;H. Aonuma
在输给对手后，雄性蟋蟀的攻击性会显著降低长达30分钟。这种攻击性的抑制是个体之间建立和维持支配地位的重要因素。在本研究中，我们研究了一氧化氮（NO）信号在雄性蟋蟀攻击性抑制中的功能作用。对雄性蟋蟀预先注射了各种no相关试剂，允许它们在二元接触中建立主导/从属关系。对手被分开15分钟，然后再次配对。在第二次交锋中，预先注射了PTIO （NO清道夫）的从属蟋蟀对前优势对手表现出拮抗行为。在预注射L-NAME （NO合成酶抑制剂）或ODQ（可溶性鸟苷酸环化酶抑制剂）的蟋蟀中观察到类似的效果。与L-NAME共注射NOR3 （NO供体）或与ODQ共注射8-Br-cGMP （cgmp -类似物）可抵消后两种药物的作用。单独注射NOR3可将从属蟋蟀的激动行为抑制从30分钟延长至3小时。我们的研究结果表明，在从属雄性蟋蟀中观察到的激动行为的变化与no介导的cGMP信号传导密切相关。
Abstract After a loss against an opponent, the aggressiveness of a male cricket is significantly reduced for up to 30 minutes. This depression of aggressiveness is an important factor in the establishment and maintenance of dominance between individuals. In the present study, we investigated the functional roles of nitric oxide (NO) signaling in the depression of aggressiveness in subordinate male crickets. Pairs of male crickets, pre-injected with various NO-related reagents, were allowed to establish dominant/subordinate relationships in dyadic encounters. Opponents were separated for 15 minutes and then paired again. In second encounters, subordinate crickets pre-injected with PTIO (NO scavenger) showed agonistic behavior towards former dominant opponents. A similar effect was observed in crickets pre-injected with L-NAME (NO synthase inhibitor) or ODQ (soluble guanylate cyclase inhibitor). The effects of the latter two drugs were canceled by co-injection of NOR3 (NO donor) with L-NAME or by co-injection of 8-Br-cGMP (cGMP-analog) with ODQ. Injection of NOR3 alone prolonged the inhibition of agonistic behavior in subordinate crickets from 30 minutes to 3 hours. Our results suggest that the change in agonistic behavior observed in subordinate male crickets is closely linked to NO-mediated cGMP signaling.