Optical imaging of the adoptive transfer of human endothelial cells in mice using anti-human CD31 monoclonal antibody
Optical imaging of the adoptive transfer of human endothelial cells in mice using anti-human CD31 monoclonal antibody
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DOI：
10.1007/s11095-006-9219-7
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发表时间：
2007-06-01
影响因子：
3.7
通讯作者：
Kang, Hye-Won
中科院分区：
文献类型：
--
作者：
Bogdanov, Alexei A., Jr.;Lin, Charles P.;Kang, Hye-Won
目的：为再生医学和癌症研究的需要，开发在流动条件下能与人细胞特异性结合的内皮特异性显像剂。本研究的目的是测试对植入小鼠体内的人内皮细胞进行光学成像的可行性。
方法：通过将细胞植入皮下空间的基质胶基质中，获得过继性人内皮细胞移植的小鼠模型（Kang、Torres、Wald、Weissleder和Bogdanov Jr.，在过继性细胞移植模型中对人内皮特异性标志物进行靶向成像。《实验室研究》86：599 - 609，2006年）。几种内皮特异性蛋白用近红外荧光染料（Cy5.5）标记，并在体外进行测试。在体内使用抗人CD31抗体进行荧光成像。通过对组织切片进行荧光显微镜检查来证实所获得的结果。
结果：我们确定用Cy5.5标记的单克隆抗人CD31抗体能有效地与人内皮细胞结合，且不会快速发生内吞作用。我们进一步证明，特异性近红外光学成像信号仅存在于接种有人内皮细胞的基质胶植入物中，而在对照基质胶植入物中不存在。组织学显示血管内皮细胞染色，并显示出CD31阳性细胞分支网络的形成。
结论：用近红外荧光染料标记的抗人CD31抗体可用于在过继性转移的动物模型中检测含有人类内皮细胞的灌注血管。
Purpose. The development of endothelium-specific imaging agents capable of specific binding to human cells under the conditions of flow for the needs of regenerative medicine and cancer research. The goal of the study was testing the feasibility of optical imaging of human endothelial cells implanted in mice.Methods. Mouse model of adoptive human endothelial cell transfer was obtained by implanting cells in Matrigel matrix in subcutaneous space (Kang, Torres, Wald, Weissleder, and Bogdanov, Jr., Targeted imaging of human endothelial-specific marker in a model of adoptive cell transfer. Lab. Invest. 86: 599609, 2006). Several endothelium-specific proteins were labeled with near-infrared fluorochrome (Cy5.5) and tested in vitro. Fluorescence imaging using anti-human CD31 antibody was performed in vivo. The obtained results were corroborated by using fluorescence microscopy of tissue sections.Results. We determined that monoclonal anti-human CD31 antibodies labeled with Cy5.5 were efficiently binding to human endothelial cells and were not subject to rapid endocytosis. We further demonstrated that specific near-infrared optical imaging signal was present only in Matrigel implants seeded with human endothelium cells and was absent from control Matrigel implants. Histology showed staining of cells lining vessels and revealed the formation of branched networks of CD31-positive cells.Conclusions. Anti-human CD31 antibodies tagged with near-infrared fluorochromes can be used for detection of perfused blood vessels harboring human endothelial cells in animal models of adoptive transfer.