Junctate is a key element in calcium entry induced by activation of InsP3 receptors and/or calcium store depletion.
Junctate is a key element in calcium entry induced by activation of InsP3 receptors and/or calcium store depletion.
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DOI：
10.1083/jcb.200404079
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发表时间：
2004-08-16
期刊：
影响因子：
--
通讯作者：
Zorzato F
中科院分区：
文献类型：
--
作者：
Treves S;Franzini-Armstrong C;Moccagatta L;Arnoult C;Grasso C;Schrum A;Ducreux S;Zhu MX;Mikoshiba K;Girard T;Smida-Rezgui S;Ronjat M;Zorzato F
在许多细胞类型中，激动剂-受体激活通过激活肌醇1，4，5三磷酸（InsP 3）受体（InsP 3Rs）导致Ca 2+从细胞内储存中快速和瞬时释放。受刺激的细胞激活位于质膜中的钙储存或受体操纵的钙通道，允许细胞外钙进入细胞质，从而补充细胞内钙储存。为了防止细胞内钙过度增加，必须精细地调节钙的进入。Junctate是内质网膜上的一种钙结合蛋白，（a）诱导和/或稳定ER和质膜之间的外周偶联，（B）与InsP 3 R和经典瞬时受体电位蛋白（TRPC）3钙进入通道形成超分子复合物。全长蛋白质调节激动剂诱导的和存储耗尽诱导的钙离子进入，而其NH 2末端影响受体激活的钙离子进入。RNA干扰耗尽细胞的内源性连接物，敲低激动剂激活的钙从细胞内储存释放和钙通过TRPC 3进入。这些结果表明，连接蛋白是一个新的蛋白质参与钙稳态的真核细胞。
In many cell types agonist-receptor activation leads to a rapid and transient release of Ca2+ from intracellular stores via activation of inositol 1,4,5 trisphosphate (InsP3) receptors (InsP3Rs). Stimulated cells activate store- or receptor-operated calcium channels localized in the plasma membrane, allowing entry of extracellular calcium into the cytoplasm, and thus replenishment of intracellular calcium stores. Calcium entry must be finely regulated in order to prevent an excessive intracellular calcium increase. Junctate, an integral calcium binding protein of endo(sarco)plasmic reticulum membrane, (a) induces and/or stabilizes peripheral couplings between the ER and the plasma membrane, and (b) forms a supramolecular complex with the InsP3R and the canonical transient receptor potential protein (TRPC) 3 calcium entry channel. The full-length protein modulates both agonist-induced and store depletion–induced calcium entry, whereas its NH2 terminus affects receptor-activated calcium entry. RNA interference to deplete cells of endogenous junctate, knocked down both agonist-activated calcium release from intracellular stores and calcium entry via TRPC3. These results demonstrate that junctate is a new protein involved in calcium homeostasis in eukaryotic cells.
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