Biosynthesis of lutropin in ovine pituitary slices: incorporation of [35S]sulfate in carbohydrate units.
Biosynthesis of lutropin in ovine pituitary slices: incorporation of [35S]sulfate in carbohydrate units.
复制标题
绵羊垂体切片中促黄体素的生物合成：在碳水化合物单元中掺入[35S]硫酸盐。
DOI：
10.1016/0003-9861(83)90458-7
复制
发表时间：
1983
影响因子：
3.9
通讯作者：
Bahl,OP
中科院分区：
文献类型：
--
作者：
Anumula,KR;Bahl,OP
Sulfate incorporation into carbohydrate of lutropin (LH) has been studied in sheep pituitary slices using H235SO4. Labeled ovine LH was purified to homogeneity by Sephadex G-100 and carboxymethyl-Sephadex chromatography from both the incubation medium and tissue extract. Autoradiography of the gel showed only two protein bands which comigrated with the α and β subunits of ovine LH in both the purified ovine LH and the immunoprecipitate obtained with LH-specific rabbit antiserum. Furthermore, [35S]sulfate was also incorporated into several other proteins in addition to LH. The location of35SO42−in the oligosaccharides of ovine LH was evidenced by its presence in the glycopeptides obtained by exhaustive Pronase digestion. The location and the point of attachment of sulfate in the carbohydrate unit were established by the isolation of 4-O-[35S]sulfo-N-acetylhexosaminyl-glycerols and 4-O-[35S]sulfo-N-acetylglucosaminitol from the Smith degradation products and by the release of35SO42−by chondro-4-sulfatase. Thus, the present line of experimentation indicates the presence of sulfate on both the terminalN-acetylglucosamine andN-acetylgalactosamine in the oligosaccharide chains of the labeled ovine LH.
