Expression dynamics of lymphoid enhancer-binding factor 1 in terminal Schwann cells, dermal papilla, and interfollicular epidermis
Expression dynamics of lymphoid enhancer-binding factor 1 in terminal Schwann cells, dermal papilla, and interfollicular epidermis
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终末雪旺细胞、真皮乳头和滤泡间表皮中淋巴增强子结合因子 1 的表达动态
DOI：
10.1002/dvdy.562
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发表时间：
2023-01-05
影响因子：
2.5
通讯作者：
Liao，Xin-Hua
中科院分区：
文献类型：
--
作者：
Song，Hongzhi;Zhao，Xu-Bo;Liao，Xin-Hua
背景转录因子淋巴增强子结合因子 1 (LEF1) 是 Wnt/β-catenin 信号通路的下游介质。它在毛囊中的真皮乳头和周围细胞中表达，促进细胞增殖和分化。结果在这里，我们报道LEF1也在终末雪旺细胞（TSC）的整个毛发周期中表达，TSC是位于峡部的披针形复合体的一个组成部分。 LEF1在峡部出现的时间与毛囊神经支配的时间一致。裸鼠异常毛囊峡部未发现LEF1。相反，TSC 中的 LEF1 是通过干细胞移植实验在裸鼠上重新构建的毛囊中发现的。皮肤去神经实验表明，TSC 中 LEF1 的表达与神经末梢无关。最后，在 T 细胞因子 3 (TCF3) 过表达的转基因小鼠中，皮肤发育早期毛囊间表皮中的 LEF1 表达显着受到抑制。结论我们揭示了皮肤发育和毛发周期中 LEF1 的表达动态。 TSC 中的 LEF1 表达表明 LEF1/Wnt 信号可能有助于在峡部区域为披针形复合体、凸起干细胞和其他邻近细胞建立一个生态位。
BackgroundTranscription factor lymphoid enhancer‐binding factor 1 (LEF1) is a downstream mediator of the Wnt/β‐catenin signaling pathway. It is expressed in dermal papilla and surrounding cells in the hair follicle, promoting cell proliferation, and differentiation.ResultsHere, we report that LEF1 is also expressed all through the hair cycle in the terminal Schwann cells (TSCs), a component of the lanceolate complex located at the isthmus. The timing of LEF1 appearance at the isthmus coincides with that of hair follicle innervation. LEF1 is not found at the isthmus in the aberrant hair follicles in nude mice. Instead, LEF1 in TSCs is found in the de novo hair follicles reconstituted on nude mice by stem cells chamber graft assay. Cutaneous denervation experiment demonstrates that the LEF1 expression in TSCs is independent of nerve endings. At last, LEF1 expression in the interfollicular epidermis during the early stage of skin development is significantly suppressed in transgenic mice with T‐cell factor 3 (TCF3) overexpression.ConclusionWe reveal the expression dynamics of LEF1 in skin during development and hair cycle. LEF1 expression in TSCs indicates that the LEF1/Wnt signal might help to establish a niche at the isthmus region for the lanceolate complex, the bulge stem cells and other neighboring cells.