Susceptibility of the domestic duck (Anas platyrhynchos) to experimental infection with Toxoplasma gondii oocysts
Susceptibility of the domestic duck (Anas platyrhynchos) to experimental infection with Toxoplasma gondii oocysts
复制标题
DOI：
10.1080/03079450310001652068
复制
发表时间：
2004-04-01
期刊：
影响因子：
2.8
通讯作者：
Literák, I
中科院分区：
文献类型：
--
作者：
Bártová, E;Dvoráková, H;Literák, I
共28只家鸭被分成7组，每组4只。分别以10(1)、10(2)、10(3)、10(4)、10(5)和10(5.7)个卵囊接种弓形虫卵囊(K21株，对小鼠无毒力)，并设对照组。在接种后第7天(P.I.)，用间接荧光抗体试验首先在所有鸭体内检测到弓形虫抗体。根据卵囊感染剂量的不同，抗体效价在1：20至1：640之间。从第14天P.I.开始抗体效价提高到1：80~1：20 480。在第14天到28天之间。(实验结束)，14只鸭的抗体效价下降，7只鸭的抗体效价不变，3只鸭的抗体效价继续上升。对小鼠的生物测定显示，弓形虫在胸肌和腿部肌肉和心脏(100%，n=47)、脑(91%，n=22)、肝脏(54%，n=13)和胃(46%，n=24)中存在。感染鸭无临床症状，但生物测定结果表明，与一些鸡科禽类相比，家鸭对弓形虫感染相对易感。
A total of 28 domestic ducks were divided into seven groups of four ducks. Six groups were inoculated per os with 10(1), 10(2), 10(3), 10(4), 10(5) and 10(5.7) oocysts Toxoplasma gondii oocysts (K21 strain, which is avirulent for mice), and the remaining group was used as a control. Antibodies to T. gondii were detected in all ducks by the indirect fluorescence antibody test first on day 7 post-inoculation (p.i.). Antibody titres were found in the range of 1:20 to 1:640 depending on the infectious dose of the oocysts. From day 14 p.i. antibody titres increased to 1:80 to 1:20 480. Between days 14 and 28 p.i. (end of the experiment), antibody titres decreased in 14 ducks, remained the same in seven ducks, and continued to increase in three ducks. Bioassay in mice revealed T. gondii in the breast and leg muscles and the heart (100%, n = 47), brain (91%, n = 22), liver (54%, n = 13) and stomach (46%, n = 24). The infected ducks showed no clinical signs; however, the results of bioassay indicate that, compared with some gallinaceous birds, domestic ducks were relatively susceptible to T. gondii infection.