A novel method for induction of plant regeneration via somatic embryogenesis
A novel method for induction of plant regeneration via somatic embryogenesis
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一种通过体细胞胚胎发生诱导植物再生的新方法
DOI：
10.1016/j.plantsci.2009.03.009
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发表时间：
2009-07-01
期刊：
影响因子：
5.2
通讯作者：
Yang, Yingwu
中科院分区：
文献类型：
--
作者：
Deng, Wei;Luo, Keming;Yang, Yingwu
为了通过体细胞胚发生诱导植株再生，构建了包含热激诱导FRT/FLP系统的二元表达载体体系。在该系统中，与AP2/ERF家族相似的BABY BOOM （BBM）基因受到花菜花叶病毒35S （CaMV 35S）启动子的控制，FLP位点特异性重组酶编码基因受到来自拟南芥的热休克诱导启动子HSP18.2的控制。启动子和基因两侧有两个直接定向的FRT重组位点。BBM过表达诱导中国白杨愈伤组织形成体胚。六个体细胞胚胎发芽形成具有表型改变的转基因植株。转基因植株的茎条经热休克处理后发育为表型正常植株。Southern blot分析进一步证实，从表型正常的转基因植株的基因组DNA中切除了BBM基因。该系统可以在不使用抗抗生素或除草剂基因的情况下获得转基因植物，并为植物体细胞胚再生提供了一种新的策略。2009爱思唯尔爱尔兰有限公司版权所有。
To induce plant regeneration via somatic embryogenesis, a binary expression vector system was constructed containing a heat shock-inducible FRT/FLP system. In this system, the BABY BOOM (BBM) gene, which shows similarity to the AP2/ERF family, was under the control of cauliflower mosaic virus 35S (CaMV 35S) promoter and the gene coding for FLP site-specific recombinase was under the control of a heat shock-inducible promoter HSP18.2 from Arabidopsis thaliana. The promoters and genes were flanked by two FRT recombination sites in direct orientation. BBM overexpression induced formation of somatic embryos from calli of Chinese white poplar (Populus tomentosa Carr.). Six somatic embryos germinated to form transgenic plants with phenotypic alterations. The stem cuttings of the transgenic plants were treated with heat shock and they developed into phenotypically normal plants. Southern blot analysis further confirmed that the BBM gene was excised from the genomic DNA of the transgenic plants with normal phenotype. The system reported here can be used to obtain transgenic plants without the use of antibiotic or herbicide resistance genes and to provide a novel strategy for plant regeneration via somatic embryogenesis. (C) 2009 Elsevier Ireland Ltd. All rights reserved.