Major Shift of Toxigenic V. cholerae O1 from Ogawa to Inaba Serotype Isolated from Clinical and Environmental Samples in Haiti
Major Shift of Toxigenic V. cholerae O1 from Ogawa to Inaba Serotype Isolated from Clinical and Environmental Samples in Haiti
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DOI：
10.1371/journal.pntd.0005045
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发表时间：
2016-10-01
影响因子：
3.8
通讯作者：
Ali, Afsar
中科院分区：
文献类型：
--
作者：
Alam, Meer T.;Ray, Shrestha S.;Ali, Afsar
2010年10月，海地一个多世纪以来首次确认爆发霍乱。一株产毒霍乱弧菌O1型El - Tor血清型小川菌株被认为是病因。在2015年10月霍乱发病5年后，我们发现了从小川血清型到稻叶血清型的重大转变（从7%到100%）。此外，利用wbeT基因测序和比较序列分析，我们现在证明，在2013年和2015年稻叶分离株中，负责将小川转化为稻叶血清型的wbeT基因持续了一个独特的核苷酸突变，而在2012年从海地获得的分离株中没有发现这种突变。此外，我们发现2015年收集的环境稻叶分离株具有与2015年临床分离株相同的突变。我们的数据表明，产毒素霍乱弧菌O1血清型Ogawa可以迅速改变其血清型稻叶，并有可能在对Ogawa血清型获得免疫的个体中引起疾病。我们的研究结果强调了在已确定有地方病的国家监测产毒素霍乱弧菌O1和霍乱的重要性。
In October of 2010, an outbreak of cholera was confirmed in Haiti for the first time in more than a century. A single clone of toxigenic Vibrio cholerae O1 biotype El Tor serotype Ogawa strain was implicated as the cause. Five years after the onset of cholera, in October, 2015, we have discovered a major switch (ranging from 7 to 100%) from Ogawa serotype to Inaba serotype. Furthermore, using wbeT gene sequencing and comparative sequence analysis, we now demonstrate that, among 2013 and 2015 Inaba isolates, the wbeT gene, responsible for switching Ogawa to Inaba serotype, sustained a unique nucleotide mutation not found in isolates obtained from Haiti in 2012. Moreover, we show that, environmental Inaba isolates collected in 2015 have the identical mutations found in the 2015 clinical isolates. Our data indicate that toxigenic V. cholerae O1 serotype Ogawa can rapidly change its serotype to Inaba, and has the potential to cause disease in individuals who have acquired immunity against Ogawa serotype. Our findings highlight the importance of monitoring of toxigenic V. cholerae O1 and cholera in countries with established endemic disease.