A spectrophotometric study of the ionizations in two ferrihaemoproteins from soya-bean nodules.
A spectrophotometric study of the ionizations in two ferrihaemoproteins from soya-bean nodules.
复制标题
大豆根瘤中两种铁血蛋白电离的分光光度研究。
DOI：
10.1042/bj0870114
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发表时间：
1963
期刊：
影响因子：
--
通讯作者：
E. Thorogood
中科院分区：
文献类型：
--
作者：
E. Thorogood
材料和方法制备颜料8.在颜料的制备过程中，温度保持在150或更低。使用块状锡蒸馏水。大豆，Glycine ma =，var.猛犸黄，提出了在莫里斯植物园，宾夕法尼亚大学。结节（800克）用0.05 μ m-磷酸盐缓冲液（pH7）均质化，可溶性提取物用硫酸铵分级。将在26 - 32%（w/w）盐浓度之间沉淀的颜料进行脱盐和浓缩。美国
MATERIALS AND METHODSPreparation of pigment8. Temperatures were held to 150 or below during the preparation of the pigments. Block-tindistilled water was used. Soya beans, Glycine ma=, var. Mammoth Yellow, were raised at the Morris Arboretum, University of Pennsylvania. Nodules (800 g.) were homo-genized in 005m-phosphate buffer, pH 7, and the soluble extract was fractionated with ammonium sulphate. The pigment which wasprecipitated between salt concentrations of 26 and 32%(w/w) was dialvsedfree of salt and concentra-* Present address: 121 South 36th Street, Philadelphia 4, Pa., USA