A Novel Reference for Bt-Resistance Mechanism in Plutella xylostella Based on Analysis of the Midgut Transcriptomes.
A Novel Reference for Bt-Resistance Mechanism in Plutella xylostella Based on Analysis of the Midgut Transcriptomes.
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DOI：
10.3390/insects12121091
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发表时间：
2021-12-07
期刊：
影响因子：
3
通讯作者：
You S
中科院分区：
文献类型：
--
作者：
Xiong L;Liu Z;Shen L;Xie C;Ye M;Li Z;Zhang Z;Li J;Dong Y;You M;You S
小菜蛾是十字花科蔬菜的重要害虫。目前，使用的防治方法主要是传统的杀虫剂和Bt作物的种植。然而，随着杀虫剂的长期和大规模使用，小菜蛾对多种杀虫剂和Bt作物产生了强烈的抗性。本文所用的小菜蛾Cry 1 S1000菌株是对Bt Cry 1Ac原毒素抗性超过8000倍的菌株。本研究利用转录组测序技术对G88敏感菌株、Cry 1 S1000抗性菌株及其相应毒素诱导菌株的中肠转录组进行了分析，以期发现更多与Bt抗性相关的基因。研究结果可为优化小菜蛾抗性防治策略、提高生物农药和Bt作物的防治效果提供参考。小菜蛾（Plutella xylostella）是一种主要危害十字花科蔬菜的鳞翅目昆虫，对包括苏云金杆菌（Bt）毒素在内的多种农用化学品具有较强的抗性。本研究旨在通过比较小菜蛾Cry 1Ac敏感品系和抗性品系以及两种毒素处理品系在采样前24 h的中肠转录组来筛选与Bt抗性相关的基因。利用BGISEQ-500对12个样本进行了分析，每个样本获得了平均6.35 Gb的数据。此外，3284差异表达基因（DEG）的敏感和耐药菌株中确定。其中，钙粘蛋白5个、氨肽酶14个、碱性磷酸酶0个、ATP结合盒转运蛋白14个和热激蛋白5个与小菜蛾对Cry 1Ac的抗性有关。此外，与“结合”、“催化活性”、“细胞过程”、“代谢过程”和“细胞解剖实体”相关的DEG更可能是Bt毒素抗性的原因。结合其他组学数据，本研究的结果将为小菜蛾的Bt抗性发展提供有前景的基因，从而为揭示小菜蛾对Bt的抗性机制提供全新的参考。
Plutella xylostella is a very serious pest to cruciferous vegetables. At present, the control methods used are mainly traditional insecticides and the cultivation of Bt crops. However, with the long-term and large-scale use of insecticides, the diamondback moth has developed strong resistance to many kinds of insecticides and Bt crops. The Cry1S1000 strain of P. xylostella used here is a strain with more than 8000 times resistance to Bt Cry1Ac protoxin. In this paper, we used transcriptome sequencing to determine the midgut transcriptome of the G88-susceptible strain, Cry1S1000-resistant strain and its corresponding toxin-induced strains to find more genes related to Bt resistance. Our results will provide a reference for optimizing the control strategy of diamondback moth resistance and improving the control efficiency of biopesticides and Bt crops. The diamondback moth, Plutella xylostella, is a lepidopteran insect that mainly harms cruciferous vegetables, with strong resistance to a variety of agrochemicals, including Bacillus thuringiensis (Bt) toxins. This study intended to screen genes associated with Bt resistance in P. xylostella by comparing the midgut transcriptome of Cry1Ac-susceptible and -resistant strains together with two toxin-treated strains 24 h before sampling. A total of 12 samples were analyzed by BGISEQ-500, and each sample obtained an average of 6.35 Gb data. Additionally, 3284 differentially expressed genes (DEGs) were identified in susceptible and resistant strains. Among them, five DEGs for cadherin, 14 for aminopeptidase, zero for alkaline phosphatase, 14 for ATP binding cassette transport, and five heat shock proteins were potentially involved in resistance to Cry1Ac in P. xylostella. Furthermore, DEGs associated with “binding”, “catalytic activity”, “cellular process”, “metabolic process”, and “cellular anatomical entity” were more likely to be responsible for resistance to Bt toxin. Thus, together with other omics data, our results will offer prospective genes for the development of Bt resistance, thereby providing a brand new reference for revealing the resistance mechanism to Bt of P. xylostella.
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影响因子：
4.5
作者：
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通讯作者：
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影响因子：
4.8
作者：
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通讯作者：
Mortazavi A
在实验室选定的小菜蛾 (L.) 菌株中，参与原毒素激活的新型中肠胰蛋白酶基因的表达减少与 Cry1Ac 抗性相关
影响因子：
4.2
作者：
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通讯作者：
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