Identification, culture, and characterization of pancreatic stellate cells in rats and humans
Identification, culture, and characterization of pancreatic stellate cells in rats and humans
复制标题
DOI：
10.1016/s0016-5085(98)70209-4
复制
发表时间：
1998-08-01
期刊：
影响因子：
29.4
通讯作者：
Adler, G
中科院分区：
文献类型：
--
作者：
Bachem, MG;Schneider, E;Adler, G
背景与目的：到目前为止，胰腺纤维化的基本基质产生细胞类型尚未确定。在这份报告中，含维甲酸的胰腺星状细胞（PSC）在大鼠和人类胰腺的描述，和肝星状细胞的形态和生化相似性。研究方法：使用胰腺组织和培养的PSC进行电子和免疫荧光显微镜检查（I型和III型胶原蛋白、纤连蛋白、层粘连蛋白、α-肌动蛋白和结蛋白）。细胞外基质合成用定量免疫分析和北方印迹分析显示。结果：PSC分布于小叶间区和腺泡间区。早期原代培养的PSC含有视黄醇和脂肪酸视黄醇酯。向传代细胞中添加视黄醇导致视黄醇摄取和酯化。在原代培养过程中，细胞从静止的脂肪储存表型变为高度合成的成肌纤维细胞样细胞，其表达iso-alpha-平滑肌肌动蛋白（> 90%）和结蛋白（20%-40%），并显示I型和III型胶原蛋白、纤连蛋白和层粘连蛋白抗体的强阳性染色。如蛋白质和信使RNA水平所确定的，血清生长因子刺激I型胶原和纤连蛋白的合成。结论：PSC的鉴定，特别是在纤维化区域，以及这些细胞与肝星状细胞的相似性表明PSC参与胰腺纤维化的发展。
Background & Aims: Until now, the basic matrix-producing cell type responsible for pancreas fibrosis has not been identified. In this report, retinoid-containing pancreatic stellate cells (PSCs) in rat and human pancreas are described, and morphological and biochemical similarities to hepatic stellate cells are shown. Methods: Electron and immunofluorescence microscopy (collagen types I and III, fibronectin, laminin, alpha-actin, and desmin) was performed using pancreatic tissue and cultured PSCs. Extracellular matrix synthesis was shown using quantitative immunoassay and Northern blot analysis. Results: PSCs are located in interlobular areas and in interacinar regions. Early primary cultured PSCs contain retinol and fatty acid retinyl-esters. Addition of retinol to passaged cells resulted in retinol uptake and esterification. During primary culture, the cells changed from a quiescent fat-storing phenotype to a highly synthetic myofibroblast-like cell expressing iso-alpha-smooth muscle actin (> 90%) and desmin (20%-40%) and showing strong positive staining with antibodies to collagen types I and Iii, fibronectin, and laminin. As determined on protein and messenger RNA level, serum growth factors stimulated the synthesis of collagen type I and fibronectin. Conclusions: The identification of PSCs, particularly in fibrotic areas, and the similarities of these cells to hepatic stellate cells suggest that PSCs participate in the development of pancreas fibrosis.