Multiple proteins interact with the fushi tarazu proximal enhancer.
Multiple proteins interact with the fushi tarazu proximal enhancer.
复制标题
多种蛋白质与 fushi tarazu 近端增强子相互作用。
DOI：
10.1128/mcb.13.9.5549-5559.1993
复制
发表时间：
1993
影响因子：
5.3
通讯作者：
Pick,L
中科院分区：
文献类型：
--
作者：
Han,W;Yu,Y;Altan,N;Pick,L
The expression of theDrosophilasegmentation genefushi tarazu(ftz) is controlled at the level of transcription. The proximal enhancer, located ~ 3.4 kb upstream of the transcription start site, directslacZfusion gene expression in aftz-like seven-stripe pattern in transgenic fly embryos. We have taken a biochemical approach to identify DNA-binding proteins that regulateftzgene expression through the proximal enhancer. DNase I footprinting and methylation interference experiments with stagedDrosophilaembryo nuclear extracts identified nine protein binding sites in the proximal enhancer. Ten different sequence-specific DNA-binding complexes that interact with eight of these sites were identified. Some interact with multiple sites, while others bind to single sites in the enhancer. Two of the complexes that interact with multiple sites appear to contain the previously describedftzregulators, FTZ-F1 and TTK/FTZ-F2. These in vitro studies allowed us to narrow down the proximal enhancer to a 323-bp DNA fragment that contains all of the protein binding sites. Expression directed by this minimal enhancer element in sevenftz-like stripes in transgenic embryos is identical to that directed by the full-length enhancer. Internal deletions of several sites abolish reporter gene expression in vivo. Thus, theftzproximal enhancer, like other cell-type-specific eukaryotic enhancers, interacts with an array of proteins that are expected to mediate the establishment, maintenance, and repression of transcription of theftzgene in seven stripes in the developing embryo.
