Role of basement membrane in EMMPRIN/CD147 induction in rat tracheal epithelial cells
Role of basement membrane in EMMPRIN/CD147 induction in rat tracheal epithelial cells
复制标题
DOI：
10.1016/j.bbrc.2008.01.105
复制
发表时间：
2008-04-04
影响因子：
3.1
通讯作者：
Nishimura, Masaharu
中科院分区：
文献类型：
--
作者：
Hosokawa, Takeshi;Betsuyaku, Tomoko;Nishimura, Masaharu
细胞外基质金属蛋白酶诱导物(EMMPRIN)是一种糖基化的跨膜蛋白，可诱导基质金属蛋白酶(MMPs)。虽然EMMPRIN的表达在生理学上仅限于胎肺上皮，但该蛋白的转录调控仍有待阐明。我们假设上皮细胞与基底膜的相互作用调节EMMPRIN的表达。基底膜具有高度整合的结构，由特定的细胞外基质组成，如层粘连蛋白和IV型胶原，并显示出多功能。我们以前开发了一种结构基底膜模拟物，合成基底膜(SBM)底物，其中掺入了胚胎肺的独特成分LN-111。在本研究中，我们定量检测了在SBM、层粘连蛋白-111、IV型胶原或层粘连蛋白-332上培养的大鼠气管上皮细胞EMMPRIN mRNA的表达。EMMPRIN在SBM和层粘连蛋白-111上表达上调，但这并不伴随着对基质金属蛋白酶-9的诱导。相反，IV型胶原和层粘连蛋白-332不能诱导EMMPRIN。这些发现提示基底膜在气管上皮EMMPRIN转录调控中的潜在作用。(C)2008 Elsevier Inc.保留所有权利。
Extracellular matrix metalloproteinase inducer (EMMPRIN) is a glycosylated transmembrane protein known to induce matrix metalloproteinases (MMPs). Although the expression of EMMPRIN is physiologically limited to fetal lung epithelium, the transcriptional regulation of this protein remains to be elucidated. We hypothesized that the interaction of epithelial cells with the basement membrane regulates EMMPRIN expression. The basement membrane has highly integrated architecture composed of specific extracellular matrix, such as laminins and type IV collagen, and exhibits multiple functions. We previously developed a structured basement membrane mimic, a synthesized basement membrane (sBM) substratum, in which laminin-111, a unique component of embryonic lungs, is incorporated. In the present study we quantified expression of EMMPRIN mRNA of rat tracheal epithelial cells cultured on sBM, laminin-111, type IV collagen, or laminin-332. EMMPRIN was upregulated on sBM and laminin-111, although this was not accompanied by MMP-9 induction. In contrast, type IV collagen and laminin-332 did not induce EMMPRIN. These findings suggest potential roles for basement membrane in the transcriptional regulation of tracheal epithelial EMMPRIN. (c) 2008 Elsevier Inc. All rights reserved.