Expression of Hepatocyte Growth Factor (HGF)/c-met during Healing Processes of Rat Experimental Gastric Ulcer
Expression of Hepatocyte Growth Factor (HGF)/c-met during Healing Processes of Rat Experimental Gastric Ulcer
复制标题
肝细胞生长因子(HGF)/c-met在大鼠实验性胃溃疡愈合过程中的表达
DOI：
10.2220/biomedres.24.19
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发表时间：
2003
影响因子：
1.2
通讯作者：
T. Shimosegawa
中科院分区：
文献类型：
--
作者：
T. Arikawa;Katsuaki Kato;H. Sasano;S. Ohara;T. Shimosegawa
肝细胞生长因子（HGF）/c-met系统被认为在胃溃疡愈合初期上皮-间质相互作用中起重要作用。然而，在胃溃疡愈合的慢性过程中，该因子的作用在很大程度上仍然未知。在这项研究中，我们研究了HGF/c-met表达的时间变化，免疫组织化学和原位杂交在乙酸诱导的大鼠胃溃疡的应用。大鼠正常胃粘膜表面上皮细胞表达c-met而不表达HGF。c-met在活动期再生上皮中表达较弱，但随着溃疡愈合的进行表达增强。活动期肉芽组织中HGF的表达不明显，但在溃疡愈合过程中，HGF的表达与c-met的表达呈一致性增强。此外，c-met表达细胞从活动期开始出现在肉芽组织中，并在愈合早期达到峰值数量，这先于HGF表达细胞的出现。在瘢痕形成期，c-met和HGF的表达强度均下降至正常胃粘膜水平。本研究结果提示HGF/c-met系统可能参与了溃疡愈合过程中再生上皮和肉芽组织的分化和成熟。
Hepatocyte growth factor (HGF)/c-met system has been thought to play an important role in the epithelial-mesenchymal interaction during the initial phase of gastric ulcer healing. However, in chronic course of gastric ulcer healing, the role of this factor remains largely unknown. In this study, we examined temporal changes of HGF/c-met expression by immunohistochemistry and in situ hybridization in an experimental ulcer induced by the application of acetic acid to the rat stomach. The rat normal gastric mucosa expressed c-met but not HGF in the surface epithelium. The expression of c-met in regenerating epithelium was weak at the active stage, but it increased the strength with the healing of ulcer progressing. The expression of HGF in the granulation tissue was undetectable at the active stage, but it increased the strength in concert with the c-met expression during the ulcer healing. In addition, c-met expressing cells appeared in granulation tissues from the active stage and reached a peak number at the early healing stage, which preceded the appearance of HGF expressing cells. At the scarring stage, expressions of both c-met and HGF decreased the intensity to the same level as normal gastric mucosa. The results of this study suggested that HGF/c-met system may work for the differentiation and maturation of regenerating epithelium and granulation tissues during ulcer healing.