THE PURIFICATION OF 4 RESPIRATORY SYNCYTIAL VIRUS PROTEINS AND THEIR EVALUATION AS PROTECTIVE AGENTS AGAINST EXPERIMENTAL-INFECTION IN BALB/C MICE
THE PURIFICATION OF 4 RESPIRATORY SYNCYTIAL VIRUS PROTEINS AND THEIR EVALUATION AS PROTECTIVE AGENTS AGAINST EXPERIMENTAL-INFECTION IN BALB/C MICE
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DOI：
10.1099/0022-1317-69-2-293
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发表时间：
1988-02-01
影响因子：
3.8
通讯作者：
TOMS, GL
中科院分区：
文献类型：
--
作者：
ROUTLEDGE, EG;WILLCOCKS, MM;TOMS, GL
用免疫亲和吸附和制备性SDS-PAGE相结合的方法，对呼吸道合胞病毒A2株的融合糖蛋白（F）、大糖蛋白（G）、磷酸化蛋白（P）和22 K蛋白进行了纯化。所有四种蛋白质在佐剂中重复接种后在小鼠中引发血清抗体，尽管通过ELISA测量的应答的幅度在小鼠与小鼠之间不同。F蛋白仅在50%的通过ELISA确定为血清阳性的小鼠中产生中和抗体。G蛋白也诱导中和抗体，但在这种情况下，中和试验和ELISA滴度更密切相关。在用P或22 K蛋白免疫的小鼠中没有检测到中和活性，尽管所有小鼠都产生了可通过ELISA检测的抗体。发现用F或G蛋白免疫的小鼠可保护其免受随后的RS病毒攻击，无论它们是否产生了中和抗体。用P或22 K蛋白接种的小鼠不受保护。
The fusion (F) glycoprotein, large-glyco- (G) protein, phospho- (P) protein and 22K protein of respiratory syncytial (RS) virus A2 strain were purified by a combination of immunoaffinity adsorption and preparative SDS-PAGE. All four proteins elicited serum antibody in mice after repeated inoculation in adjuvant, although the magnitude of the response as measured by ELISA varied from mouse to mouse. The F protein generated neutralizing antibodies in only 50% of the mice determined to be seropositive by ELISA. The G protein also induced neutralizing antibodies but in this instance neutralization tests and ELISA titres were more closely correlated. No neutralizing activity was detected in mice immunized with the P or 22K proteins although all produced antibody detectable by ELISA. Mice immunized with either the F or the G protein were found to be protected against subsequent RS virus challenge, whether they had developed neutralizing antibody or not. Mice inoculated with the P or 22K proteins were not protected.