Transcutaneous measurement of renal function in conscious mice
Transcutaneous measurement of renal function in conscious mice
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DOI：
10.1152/ajprenal.00279.2012
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发表时间：
2012-09-01
影响因子：
4.2
通讯作者：
Schock-Kusch, Daniel
中科院分区：
文献类型：
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作者：
Schreiber, Andrea;Shulhevich, Yury;Schock-Kusch, Daniel
Schreiber A，Shulhevich Y，Geraci S，Hesser J，Stsepankou D，Neudecker S，Koenig S，Heinrich R，Hoecklin F，Pill J，Friedemann J，Schweda F，Gretz N，Schock-Kusch D.清醒小鼠肾功能的经皮测量。美国肾脏生理学杂志303：F783-F788，2012年。首次发表于2012年6月13日; doi：10.1152/ajprenal.00279.2012.-在清醒的小鼠中测定肾小球滤过率（GFR）对于实验者来说是繁琐的，并且对动物来说是有压力的。在这里，我们报告了一个简单的新技术，允许经皮测量GFR在清醒的小鼠。这种方法将我们以前开发的大鼠技术扩展到小鼠。该技术依赖于配备有内部存储器的小型化设备，该内部存储器允许经皮测量荧光肾脏标记物FITC-左己烯雌酚的消除动力学。该装置进行了描述和验证，与健康的，单侧肾切除和pcy小鼠的FITC-左司他丁血浆清除率相比。总之，我们描述了一种技术，允许自由活动的小鼠的肾功能的测量独立的血液或尿液采样以及实验室测定。
Schreiber A, Shulhevich Y, Geraci S, Hesser J, Stsepankou D, Neudecker S, Koenig S, Heinrich R, Hoecklin F, Pill J, Friedemann J, Schweda F, Gretz N, Schock-Kusch D. Transcutaneous measurement of renal function in conscious mice. Am J Physiol Renal Physiol 303: F783-F788, 2012. First published June 13, 2012; doi: 10.1152/ajprenal.00279.2012.-Determination of glomerular filtration rate (GFR) in conscious mice is cumbersome for the experimenter and stressful for the animals. Here we report on a simple new technique allowing the transcutaneous measurement of GFR in conscious mice. This approach extends our previously developed technique for rats to mice. The technique relies on a miniaturized device equipped with an internal memory that permits the transcutaneous measurement of the elimination kinetics of the fluorescent renal marker FITC-sinistrin. This device is described and validated compared with FITC-sinistrin plasma clearance in healthy, unilaterally nephrectomized and pcy mice. In summary, we describe a technique allowing the measurement of renal function in freely moving mice independent of blood or urine sampling as well as of laboratory assays.