Antimicrobial Activity and Prevention of Bacterial Biofilm Formation of Silver and Zinc Oxide Nanoparticle-Containing Polyester Surfaces at Various Concentrations for Use
Antimicrobial Activity and Prevention of Bacterial Biofilm Formation of Silver and Zinc Oxide Nanoparticle-Containing Polyester Surfaces at Various Concentrations for Use
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DOI：
10.3390/foods9040442
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发表时间：
2020-04-01
期刊：
影响因子：
5.2
通讯作者：
Juan Rodriguez-Jerez, Jose
中科院分区：
文献类型：
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作者：
Fontecha-Umana, Fabio;Guillermo Rios-Castillo, Abel;Juan Rodriguez-Jerez, Jose
食品接触表面是食品工业过程中细菌污染的主要来源。为了防止表面上的细菌粘附和生物膜形成，本研究评估了含有银（Ag-NPs）和氧化锌（ZnO-NPs）纳米颗粒的聚酯表面（浓度范围为400 ppm至850 ppm）对革兰氏阳性（金黄色葡萄球菌）和革兰氏阴性（大肠杆菌）两种细菌的抗菌活性，以及利用病原体单核细胞增生李斯特氏菌预防细菌生物膜形成的效果。抗菌功效结果（减少量 >= 2 log CFU/cm(2)）表明，浓度为 850 ppm 时，ZnO-NP 仅对大肠杆菌有效（2.07 log CFU/cm(2)）。然而，浓度为 400 ppm 的 Ag-NP 可有效对抗大肠杆菌 (4.90 log CFU/cm(2)) 和金黄色葡萄球菌 (3.84 log CFU/cm(2))。此外，850 ppm Ag-NPs 和 400 ppm ZnO-NPs 的组合浓度对大肠杆菌 (5.80 log CFU/cm(2)) 和金黄色葡萄球菌 (4.11 log CFU/cm(2)) 表现出较高的抗菌功效。结果还表明，Ag-ZnO-NP 的浓度水平与细菌活性之间存在高度相关性（金黄色葡萄球菌的 R-2 = 0.97，大肠杆菌的 R-2 = 0.99）。他们还表明，与单独作用不同，Ag-NPs 和 ZnO-NPs 的联合作用对两种类型的微生物都具有很高的抗菌功效。此外，Ag-NPs 在浓度为 500 ppm 的潮湿生长条件下可防止单核细胞增生李斯特菌生物膜的形成。需要在不同条件下进行更多研究来测试含有纳米粒子且具有抗菌特性的聚酯表面的耐久性，以优化其使用。
Food contact surfaces are primary sources of bacterial contamination in food industry processes. With the objective of preventing bacterial adhesion and biofilm formation on surfaces, this study evaluated the antimicrobial activity of silver (Ag-NPs) and zinc oxide (ZnO-NPs) nanoparticle-containing polyester surfaces (concentration range from 400 ppm to 850 ppm) using two kinds of bacteria, Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli), and the prevention of bacterial biofilm formation using the pathogen Listeria monocytogenes. The results of antimicrobial efficacy (reductions >= 2 log CFU/cm(2)) showed that at a concentration of 850 ppm, ZnO-NPs were effective against only E. coli (2.07 log CFU/cm(2)). However, a concentration of 400 ppm of Ag-NPs was effective against E. coli (4.90 log CFU/cm(2)) and S. aureus (3.84 log CFU/cm(2)). Furthermore, a combined concentration of 850 ppm Ag-NPs and 400 ppm ZnO-NPs showed high antimicrobial efficacy against E. coli (5.80 log CFU/cm(2)) and S. aureus (4.11 log CFU/cm(2)). The results also showed a high correlation between concentration levels and the bacterial activity of Ag-ZnO-NPs (R-2 = 0.97 for S. aureus, and R-2 = 0.99 for E. coli). They also showed that unlike individual action, the joint action of Ag-NPs and ZnO-NPs has high antimicrobial efficacy for both types of microorganisms. Moreover, Ag-NPs prevent the biofilm formation of L. monocytogenes in humid conditions of growth at concentrations of 500 ppm. Additional studies under different conditions are needed to test the durability of nanoparticle containing polyester surfaces with antimicrobial properties to optimize their use.