Preferential transposition of Drosophila P elements to nearby chromosomal sites.
Preferential transposition of Drosophila P elements to nearby chromosomal sites.
复制标题
果蝇 P 元件优先转座到附近的染色体位点。
DOI：
10.1093/genetics/133.2.347
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发表时间：
1993
期刊：
影响因子：
3.3
通讯作者：
Spradling,AC
中科院分区：
文献类型：
--
作者：
Tower,J;Karpen,GH;Craig,N;Spradling,AC
两种不同的方案被用来证明果蝇P元件优先转座到基因组区域接近其起始位点。具有弱玫瑰色+标记基因表达的起始元件从其在1，300-kb Dp 1187微型染色体的亚端粒区域中的位置被动员。在具有改变的rosy+表达的后代系中，远高于预期的频率在Dp 1187上包含新的插入。终末缺陷也经常恢复。在第二次筛选中，在雄性和雌性生殖系中动员了引起仙人掌基因致死突变的玫瑰色（+）标记的元件，并回收了由于染色体内转座而仍然含有玫瑰色+基因的可行回复突变体染色体。使用这两种方法回收的新的转座被映射在从起始位点的0和128 kb之间。我们的研究结果表明，某些机制提高了频率43-67倍，其中一个P元素插入其起始位点附近。局部转座可能有助于提高基因组预定区域内的插入突变率。
Two different schemes were used to demonstrate that Drosophila P elements preferentially transpose into genomic regions close to their starting sites. A starting element with weak rosy+ marker gene expression was mobilized from its location in the subtelomeric region of the 1,300-kb Dp1187 minichromosome. Among progeny lines with altered rosy+ expression, a much higher than expected frequency contained new insertions on Dp1187. Terminal deficiencies were also recovered frequently. In a second screen, a rosy(+)-marked element causing a lethal mutation of the cactus gene was mobilized in male and female germlines, and viable revertant chromosomes were recovered that still contained a rosy+ gene due to an intrachromosomal transposition. New transpositions recovered using both methods were mapped between 0 and 128 kb from the starting site. Our results suggested that some mechanism elevates the frequency 43-67-fold with which a P element inserts near its starting site. Local transposition is likely to be useful for enhancing the rate of insertional mutation within predetermined regions of the genome.