Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution
Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution
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DOI：
10.1126/science.276.5314.930
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发表时间：
1997-05-09
期刊：
影响因子：
56.9
通讯作者：
Griffin, RG
中科院分区：
文献类型：
--
作者：
Hall, DA;Maus, DC;Griffin, RG
在冷冻甘油-水溶液中，精氨酸和蛋白质T4溶菌酶的固体魔角旋转核磁共振谱利用动态核极化获得了大的动态核极化信号增强(高达100倍)。在电子顺磁共振频率附近，通过微波辐射，将氮氧自由基的未配对电子的极化转移到核自旋。这种方法可能是一种普遍适用于生物分子高分辨率固体核磁共振谱的信号增强方案。
Large dynamic nuclear polarization signal enhancements (up to a factor of 100) were obtained in the solid-state magic-angle spinning nuclear magnetic resonance (NMR) spectra of arginine and the protein T4 lysozyme in frozen glycerol-water solutions with the use of dynamic nuclear polarization. Polarization was transferred from the unpaired electrons of nitroxide free radicals to nuclear spins through microwave irradiation near the electron paramagnetic resonance frequency. This approach may be a generally applicable signal enhancement scheme for the high-resolution solid-state NMR spectroscopy of biomolecules.