Optical properties of tissues quantified using morphological granulometry from phase-contrast images of thin tissue samples.
Optical properties of tissues quantified using morphological granulometry from phase-contrast images of thin tissue samples.
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DOI：
10.3233/xst-140474
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发表时间：
2015
影响因子：
3
通讯作者：
Chen WR
中科院分区：
文献类型：
--
作者：
Li Z;Chen H;Li H;Chen WR
提出了一种结合形态粒度法和米氏理论测定生物组织光散射的方法。采用Otsu方法对相衬图像进行二值化处理。基于二值相衬图像，采用二值形态粒度法估计组织样品的大小密度分布。结果表明，将尺寸密度分布与米氏理论相结合，可以定量地确定与组织中光散射相关的光学参数。这表明，这种独特的方法可以用来表征疾病诊断的生物组织。
A method combining morphological granulometry with Mie theory to determine optical scattering in biological tissues was proposed. Otsu’s method was applied to binarize phase-contrast images. Binary morphological granulometry was used to estimate size density distribution of the tissue samples based on the binary phase-contrast images. Our results showed that the optical parameters associated with light scattering in tissue could be quantitatively determined by combining size density distribution with Mie theory. It was suggested that this unique method could be used to characterize biological tissues for disease diagnosis.