Population genetic structure and mycotoxin potential of the wheat crown rot and head blight pathogen Fusarium culmorum in Algeria
Population genetic structure and mycotoxin potential of the wheat crown rot and head blight pathogen Fusarium culmorum in Algeria
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DOI：
10.1016/j.fgb.2017.04.001
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发表时间：
2017-06-01
影响因子：
3
通讯作者：
O'Donnell, Kerry
中科院分区：
文献类型：
--
作者：
Laraba, Imane;Botreghda, Houda;O'Donnell, Kerry
2014年和2015年对阿尔及利亚小麦冠腐病（FCR）和白头病（FHB）的调查显示，产生3-乙酰脱氧雪腐镰刀菌（Fusarium culmorum）或雪腐镰刀菌醇（nivalol）的菌株是这两种重要病害的致病因子。菌株的形态学鉴定（n FCR = 110, n FHB = 30）通过测序部分TEF1得到证实。为评价阿尔及利亚菌株的交偶型、毛线虫化学型潜能和全球种群结构，将阿尔及利亚菌株与意大利（rt = 27）、澳大利亚（n = 30）和美国（n = 28）的culmorum F.初步样本进行比较。MAT1-1和MAT1-2菌株的分离比例几乎相等，但在阿尔及利亚，三分之二的菌株是MAT1-2。等位基因特异性PCR分析表明，3ADON毛线虫基因型在全球占主导地位（83.8% 3ADON），在四个抽样国家均占主导地位。体外毒素分析证实了毛霉菌基因型PCR数据，并表明大多数测试菌株（77%）产生culmorin。利用9个微卫星标记（SSRs）对191个菌株的全球群体遗传结构进行了评估。对克隆校正数据的AMOVA分析表明，89%的变异发生在种群内。SSR数据的贝叶斯分析鉴定出两个全球分布的同域居群，其中既有毛藻化学型，也有交配型。
Surveys for crown rot (FCR) and head blight (FHB) of Algerian wheat conducted during 2014 and 2015 revealed that Fusarium culmorum strains producing 3-acetyl-deoxynivalenol (3ADON) or nivalenol (NIV) were the causal agents of these important diseases. Morphological identification of the isolates (n FCR = 110, n FHB = 30) was confirmed by sequencing a portion of TEF1. To assess mating type idiomorph, trichothecene chemotype potential and global population structure, the Algerian strains were compared with preliminary sample of F. culmorum from Italy (rt = 27), Australia (n = 30) and the United States (n = 28). A PCR assay for MAT idiomorph revealed that MAT1-1 and MAT1-2 strains were segregating in nearly equal proportions, except within Algeria where twothirds of the strains were MAT1-2. An allele-specific PCR assay indicated that the 3ADON trichothecene genotype was predominant globally (83.8% 3ADON) and in each of the four countries sampled. In vitro toxin analyses confirmed trichothecene genotype PCR data and demonstrated that most of the strains tested (77%) produced culmorin. Global population genetic structure of 191 strains was assessed using nine microsatellite markers (SSRs). AMOVA of the clone corrected data indicated that 89% of the variation was within populations. Bayesian analysis of the SSR data identified two globally distributed, sympatric populations within which both trichothecene chemotypes and mating types were represented.