Impact of nicotine on bone healing.
Impact of nicotine on bone healing.
复制标题
尼古丁对骨愈合的影响。
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
D. Buck
中科院分区：
文献类型：
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作者：
Jeffrey O. Hollinger;John M. Schmitt;Kun Hwang;Peiman Soleymani;D. Buck
有限数量的实验动物研究和体外数据证实，尼古丁损害骨愈合，减少成骨细胞功能，导致自体骨移植物发病，并降低移植物生物力学性能。因此，我们的长期目标是开发一种有效的治疗方法来逆转烟草产品中尼古丁的不良影响。然而，在实现这一目标之前，我们必须开发一种动物模型。我们的假设是，在一个特征性的动物伤口模型中，在自体骨移植前后给予尼古丁会对自体骨移植物的结合产生不利影响，并抑制供体部位的愈合。在60只Long-Evans大鼠（雄性，35日龄）制备的双侧4 mm直径顶骨缺损中完成假设检验。从左侧顶骨中取出一个直径为4 mm的供体骨圆盘，并将其放置在对侧缺损处。供体部位作为自发愈合的骨伤口。将大鼠平均分配至三种剂量的尼古丁（12.5、25和50 mg/L）经口饮水给药。对于每个剂量，尼古丁治疗的持续时间和顺序遵循四个疗程，包括无尼古丁和指定的尼古丁给药组合以及骨手术前后的减量。在术后14天和28天恢复实验部位，定量反应，并通过方差分析和事后统计分析数据（p <或= 0.05）。我们开发了一种方便有效的骨模型，结果验证了我们的假设，尼古丁对骨愈合有负面影响。
A limited number of experimental animal studies and in vitro data confirm that nicotine impairs bone healing, diminishes osteoblast function, causes autogenous bone graft morbidity, and decreases graft biomechanical properties. Therefore, our long-term goal is to develop an effective therapy to reverse the adverse impact of nicotine from tobacco products. However, before accomplishing this goal, we had to develop an animal model. Our hypotheses were nicotine administration preceding and following autogenous bone grafting adversely affected autograft incorporation and depressed donor site healing in a characterized animal wound model. Hypothesis testing was accomplished in bilateral, 4-mm diameter parietal bone defects prepared in 60 Long-Evans rats (male, 35-day-old). A 4-mm diameter disk of donor bone was removed from the left parietal bone and placed in the contralateral defect. The donor site served as a spontaneously healing bone wound. The rats were partitioned equally among three doses of nicotine administered orally in the drinking water (12.5, 25, and 50 mg/L). For each dose, the duration and sequence of nicotine treatment followed four courses, including no nicotine and designated combinations of nicotine administration and abatement prior to and following osseous surgery. Experimental sites were recovered on 14 and 28 days postsurgery, responses quantitated, and data analyzed by analysis of variance and post hoc statistics (p < or = 0.05). We developed a convenient and effective osseous model, and the results validated our hypothesis that nicotine negatively impacts on bone healing.
影响因子：
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作者：
Jensen Ja;W. Goodson;H. Hopf;T. K. Hunt
通讯作者：
Jensen Ja;W. Goodson;H. Hopf;T. K. Hunt