Comparison of phosphatidylcholines containing one or two docosahexaenoic acyl chains on properties of phospholipid monolayers and bilayers.
Comparison of phosphatidylcholines containing one or two docosahexaenoic acyl chains on properties of phospholipid monolayers and bilayers.
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含有一个或两个二十二碳六烯酰基链的磷脂酰胆碱对磷脂单层和双层性质的比较。
DOI：
10.1016/0005-2736(95)00058-b
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Stillwell,W
中科院分区：
文献类型：
--
作者：
Zerouga,M;Jenski,LJ;Stillwell,W
二十二碳六烯酸是膜中发现的n-−-3脂肪酸中最长和最不饱和的。尽管已经证明这种脂肪酸的存在会影响膜的一些性质，但它的作用方式还没有被清楚地阐明。以前关于生物膜的报道没有区分单二十二碳六烯酰磷脂和那些在两条链上都含有二十二碳六烯酸的磷脂的作用。本报告比较了由1-stearoyl-2-linolenoyl-sn-glycero-3-phosphocholine(作为对照)、1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine或1，2-di-docosahexaenoyl-sn-glycero-3-phosphocholine.组成的单层膜和双层膜的性质与单DHA磷脂酰胆碱(PC)相比，二DHA磷脂酰胆碱(PC)占据了更大的面积/分子，支持更流动和更具渗透性的双层，并且不容易被过氧化。由任何一种磷脂制成的单分子膜都不能被胆固醇凝结。我们认为，许多与DHA相关的膜性质可能是由于磷脂失去了正常的位置选择性而将DHA结合到这两个位置上的结果。
Docosahexaenoic acid (DHA) is the longest and most unsaturated of the n − 3 fatty acids found in membranes. Although a number of membrane properties have been demonstrated to be affected by the presence of this fatty acid, its mode of action has yet to be clearly elucidated. Prior reports on biological membranes have not distinguished the effect of mono-docosahexaenoyl phospholipids from those caused by phospholipids containing docosahexaenoic acid in both chains. This report compares properties of monolayers and bilayers composed of either 1-stearoyl-2-linolenoyl-sn-glycero-3-phosphocholine (as a control), 1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine or 1,2-di-docosahexaenoyl-sn-glycero-3-phosphocholine. When compared to the mono-DHA phosphatidylcholine (PC), the di-DHA PC occupies a much larger area/molecule, supports a more fluid and permeable bilayer, and is less susceptible to peroxidation. Monolayers made from either phospholipid are not condensable by cholesterol. We suggest many of the membrane properties linked to the presence of DHA may be the result of phospholipids which have lost their normal positional selectivity and have incorporated DHA into both positions.