In Vitro Biological Characterization of DCUN1D5 in DNA Damage Response
In Vitro Biological Characterization of DCUN1D5 in DNA Damage Response
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DCUN1D5 在 DNA 损伤反应中的体外生物学表征
DOI：
10.7314/apjcp.2012.13.8.4157
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发表时间：
2012-01-01
影响因子：
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通讯作者：
Huang, Zhi-Gang
中科院分区：
文献类型：
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作者：
Guo, Wei;Li, Guo-Jun;Huang, Zhi-Gang
背景：喉鳞状细胞癌（LSCC）新的预后生物标志物或治疗分子靶点是一个紧迫的优先事项。我们在这里试图确定多个新的LSCC相关基因。研究方法：使用高密度微阵列表达谱，我们确定了多个基因，显着改变人类LSCC和配对正常组织之间。一个这样的基因，DCUN1D5，潜在的致癌功能，进一步在体外进行了表征。结果：DCUN1D5在LSCC中高表达。在体外过表达DCUN1D5导致细胞迁移增加2.7倍，侵袭能力增加67.5%，增殖增加2.6倍。内源性DCUN1D5表达在基因毒性应激后以时间依赖性方式降低，并且通过siRNA沉默DCUN1D5使S期细胞数量减少10.2%，凋亡增加11.7%。结论：我们的数据表明，DCUN 1D5在体外可能在DNA损伤反应中起重要作用，但需要进一步研究以评估其在体内的意义。
Background: Novel prognostic biomarkers or therapeutic molecular targets for laryngeal squamous cell carcinoma (LSCC) are an urgent priority. We here sought to identify multiple novel LSCC-associated genes. Methods: Using high-density microarray expression profiling, we identified multiple genes that were significantly altered between human LSCCs and paired normal tissues. Potential oncogenic functions of one such gene, DCUN1D5, were further characterized in vitro. Results: Our results demonstrated that DCUN1D5 was highly expressed in LSCCs. Overexpression of DCUN1D5 in vitro resulted in 2.7-fold increased cellular migration, 67.5% increased invasive capacity, and 2.6-fold increased proliferation. Endogenous DCUN1D5 expression was decreased in a time-dependent manner after genotoxic stress, and silencing of DCUN1D5 by siRNA decreased the number of cells in the S phase by 10.2% and increased apoptosis by 11.7%. Conclusion: Our data suggest that DCUN1D5 in vitro might have vital roles in DNA damage response, but further studies are warranted to assess its significance in vivo.