A IncRNA-H19 transcript from secondary hair follicle of Liaoning cashmere goat: Identification, regulatory network and expression regulated potentially by its promoter methylation
A IncRNA-H19 transcript from secondary hair follicle of Liaoning cashmere goat: Identification, regulatory network and expression regulated potentially by its promoter methylation
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辽宁绒山羊次级毛囊的IncRNA-H19转录物：鉴定、调控网络和可能受其启动子甲基化调控的表达
DOI：
10.1016/j.gene.2017.10.028
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发表时间：
2018
期刊：
影响因子：
3.5
通讯作者：
Bai Wen L
中科院分区：
文献类型：
--
作者：
Zhu Yu B;Wang Ze Y;Yin Rong H;Jiao Qian;Zhao Su J;Cong Yu Y;Xue Hui L;Guo Dan;Wang Shi Q;Zhu Yan X;Bai Wen L
H19转录本（印迹母源表达转录本）是众所周知的长链非编码RNA（lncRNA），它被认为与毛乳头细胞毛囊重建的诱导能力有关。本研究从辽宁绒山羊次级毛囊中分离并鉴定了lncRNA-H19转录本。此外，我们还研究了H19基因在该品种次级毛囊中的转录模式和甲基化状态在毛囊周期的不同阶段。核苷酸组成分析表明，辽宁绒山羊lncRNA-H19转录本中的优势核苷酸为鸟嘌呤（G）和胞嘧啶（C），其中GG核苷酸对的频率最高（11.25%）。调控网络显示lncRNA-H19转录本与其相关的miRNAs和潜在的靶基因之间存在着非常多样的调控关系。在次级毛囊中，lncRNA-H19转录本在生长期的相对表达量显著高于休止期和退化期，提示lncRNA-H19转录本可能在山羊绒纤维的形成和生长中起重要作用。甲基化分析表明H19基因启动子区甲基化可能参与了H19基因在辽宁绒山羊次级毛囊中的转录抑制。
The H19 transcript (imprinted maternally expressed transcript) is well-known as long noncoding RNA (lncRNA), and it is thought to be associated with the inductive capacity of dermal papilla cells for hair-follicle reconstruction. In this study, we isolated and characterized a lncRNA-H19 transcript from the secondary hair follicle of Liaoning cashmere goat. Also, we investigated its transcriptional pattern and methylation status of H19 gene in secondary hair follicle of this breed during different stages of hair follicle cycle. Nucleotide composition analysis indicated that guanine (G) and cytosine (C) are the dominant nucleotides in the lncRNA-H19 transcript of Liaoning cashmere goat with the highest frequency distribution (11.25%) of GG nucleotide pair. The regulatory network showed that lncRNA-H19 transcript appears to have remarkably diverse regulatory relationships with its related miRNAs and the potential target genes. In secondary hair follicle, the relative expression of lncRNA-H19 transcript at the anagen phase is significantly higher than that at both telogen and catagen phases suggesting that lncRNA-H19 transcript might play essential roles in the formation and growth of cashmere fiber of goat. Methylation analysis indicated that the methylation of the promoter region of H19 gene most likely participates in its transcriptional suppression in secondary hair follicle of Liaoning cashmere goat.