Genetic dissection of the tissue-specific roles of type III effectors and phytotoxins in the pathogenicity of Pseudomonas syringae pv. syringae to cherry
Genetic dissection of the tissue-specific roles of type III effectors and phytotoxins in the pathogenicity of Pseudomonas syringae pv. syringae to cherry
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III 型效应子和植物毒素在丁香假单胞菌致病性中的组织特异性作用的基因剖析。
DOI：
10.1101/2024.02.06.578989
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发表时间：
2024
期刊：
影响因子：
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通讯作者：
Vadillo-Dieguez A
中科院分区：
文献类型：
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作者：
Vadillo-Dieguez A
与假单胞菌复合体中的其它类群相比，假单胞菌P. taginagaepv. PG 2内的Pss菌株具有减少的III型效应子（T3 Es）库，但产生几种植物毒素。樱桃病原菌Pss 9644内的效应子根据其在来自李属的菌株中的频率进行分组，因为大多数Pss 9644共有的保守效应子位点（CEL）。樱桃病原体共有的一组PRUNUS效应子; PG 2共有的一组核心效应子;樱桃病原体共有的一组PRUNUS效应子;以及一组灵活的T3 E。Pss 9644还含有用于毒素黄曲霉霉素、黄曲霉肽素和黄曲霉素A的生物合成的基因簇。在确认毒力基因表达后，突变体与一系列的T3 E和毒素缺失的致病性测试的木材，叶片和果实的甜樱桃（甜樱桃）和叶片的观赏樱桃（樱桃incisa）。毒素在水果病害发展中起关键作用，但在叶和木材中不太重要。一个无效突变体保留了一定的致病性水果，但不是木材或树叶。在效应子组中观察到显著的冗余。CEL效应子在叶片感染的早期阶段具有重要作用，并且可能与所有组织中的毒素协同作用。T_3E的缺失对P的影响更大。在P. avium混合接种物用于补充毒素突变的反式，并表明菌株混合物在田间可能是重要的。我们的结果突出了毒素在P中的生态位特异性作用。aviumtissues和病原体Pss 9644中效应冗余的复杂性。
When compared with other phylogroups (PGs) of thePseudomonas syringaespecies complex,P. syringaepv. syringae(Pss) strains within PG2 have a reduced repertoire of type III effectors (T3Es) but produce several phytotoxins. Effectors within the cherry pathogen Pss 9644 were grouped based on their frequency in strains fromPrunusas the conserved effector locus (CEL) common to mostP. syringaepathogens; a core of effectors common to PG2; a set of PRUNUS effectors common to cherry pathogens; and a FLEXIBLE set of T3Es. Pss 9644 also contains gene clusters for biosynthesis of toxins syringomycin, syringopeptin and syringolin A. After confirmation of virulence gene expression, mutants with a sequential series of T3E and toxin deletions were pathogenicity tested on wood, leaves and fruits of sweet cherry (Prunus avium) and leaves of ornamental cherry (Prunus incisa). The toxins had a key role in disease development in fruits but were less important in leaves and wood. An effectorless mutant retained some pathogenicity to fruit but not wood or leaves. Striking redundancy was observed amongst effector groups. The CEL effectors have important roles during the early stages of leaf infection and possibly acted synergistically with toxins in all tissues. Deletion of separate groups of T3Es had more effect inP. incisathan inP. avium. Mixed inocula were used to complement the toxin mutations in trans and indicated that strain mixtures may be important in the field. Our results highlight the niche‐specific role of toxins inP. aviumtissues and the complexity of effector redundancy in the pathogen Pss 9644.