Characterization of uniaxially aligned chitin film by 2D FT-IR spectroscopy.
Characterization of uniaxially aligned chitin film by 2D FT-IR spectroscopy.
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DOI：
10.1021/bm0492172
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发表时间：
2005-05
影响因子：
6.2
通讯作者：
Y. Yamaguchi;T. Nge;Akio Takemura;Naruhito Hori;H. Ono
中科院分区：
文献类型：
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作者：
Y. Yamaguchi;T. Nge;Akio Takemura;Naruhito Hori;H. Ono
利用二维傅里叶变换红外光谱（2D FT-IR）研究了甲壳素的结晶结构。从这项研究中，获得了在传统的1D FT-IR中没有观察到的光谱带的新信息。在2D光谱中，在OH区域的3482、3421和3380 cm（-1）处区分出三个特异性条带。它们可以被指定为C（6）OH基团，其与下一个C（6）OH氢键键合; C（3）OH基团与O（5）氢键键合;以及C（6）OH基团分别与C（6）OH和C=O氢键键合。酰胺区出现两对谱带，表明C=O基团的两种氢键状态。这与OH区域的结果非常一致;一半的C（6）OH基团与C=O以及下一个C（6）OH形成氢键。结果准确地证实了Blackwell模型，这是建立了一个X-射线衍射研究。通过二维红外光谱（2DFT-IR）研究了氢键的温度依赖性，发现链间氢键[C（6）OH]. O（6）]首先响应温度，然后是链内[C（6）OH. O=C]和[C（3）OH. O（5）]随温度的升高。链间氢键[NH. O=C]在40-180 ℃的温度范围内相对稳定。
Two-dimensional FT-IR spectroscopy (2D FT-IR) was applied to the investigations of crystalline chitin structure. From this study, new information about spectral bands which are not observed in conventional 1D FT-IR was obtained. In 2D spectra, three specific bands were differentiated at 3482, 3421, and 3380 cm(-1) in the OH region. They could be assigned as C(6)OH groups which are hydrogen-bonded to the next C(6)OH; C(3)OH groups hydrogen-bonded to O(5); and C(6)OH groups bifurcated hydrogen-bonded to C(6)OH as well as C=O, respectively. Two pairs of bands appeared in the amide region, indicating the two types of hydrogen-bonded states of C=O groups. This is in good agreement with the results in the OH region; half of the C(6)OH groups are hydrogen-bonded to C=O as well as the next C(6)OH. The results accurately confirmed the Blackwell model which was established by an X-ray diffraction study. The temperature-dependency of hydrogen-bonds was also revealed by 2D FT-IR. Interchain hydrogen-bonds [C(6)OH...O(6)] first respond to a temperature followed by intrachain [C(6)OH...O=C] and [C(3)OH...O(5)] with increasing temperature. Interchain hydrogen-bonds [NH...O=C] are relatively stable in the temperature range of 40-180 degrees C.