Dynamic response of Aspergillus niger to single pulses of glucose with high and low concentrations
Dynamic response of Aspergillus niger to single pulses of glucose with high and low concentrations
复制标题
黑曲霉对高、低浓度葡萄糖单脉冲的动态响应
DOI：
10.1186/s40643-019-0251-y
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发表时间：
2019-05
影响因子：
4.6
通讯作者：
Noorman Henk
中科院分区：
文献类型：
--
作者：
Wang Shuai;Liu Peng;Shu Wei;Li Chao;Li Huan;Liu Shanshan;Xia Jianye;Noorman Henk
微生物通常在其自然栖息地遇到波动的环境，类似的条件也发生在大型生物反应器中。在这项工作中，细胞内和细胞外代谢产物的尼日尔的动态响应后，突然暴露于高和低过量葡萄糖浓度的恒化器进行了研究。结果发现，通过中央碳代谢（CCM）的碳通量的稳态途径周转时间为PP途径50 s，EMP途径20 s，TCA循环189 s，并估计CCM中的单个代谢物浓度的上限。无论葡萄糖脉冲大小如何，观察到除了天冬氨酸外的氨基酸水平几乎没有变化，天冬氨酸显示出显著降低。在所研究的A.尼日尔菌株。然而，在A中发现NAD+/NADH比率对葡萄糖脉冲的不同响应。与先前发表的对产黄青霉和酿酒酵母的观察结果相比，这些发现对于更好地理解A.在大规模生物反应器中的黑尼日尔培养性能。
Microorganisms generally encounter a fluctuating environment in their natural habitat and similar conditions also happen in large-scale bioreactors. In this work, the dynamic response of intracellular and extracellular metabolites of Aspergillus niger was investigated after sudden exposure to high and low excess glucose concentrations in chemostats. It was found that the steady-state pathway turnover time of the carbon flux through the central carbon metabolism (CCM) was PP pathway 50 s, EMP pathway 20 s, and TCA cycle 189 s, and an upper limit for individual metabolite concentrations in the CCM was estimated. Regardless of the glucose pulse size, little changes of amino acids levels were observed except for aspartate, which showed a significant decrease. The ATP paradox, known from other organisms, was also observed in the studied A. niger strain. However, a different response of the NAD+/NADH ratio to the glucose pulses was found in A. niger compared to previously published observations on Penicillium chrysogenum and Saccharomyces cerevisiae. These findings are valuable for better understanding A. niger culture performance in large-scale bioreactors.
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影响因子：
4.1
作者：
Suarez-Mendez CA;Sousa A;Heijnen JJ;Wahl A
通讯作者：
Wahl A
影响因子：
4.4
作者：
Liang Wu;J. V. van Dam;D. Schipper;M. Kresnowati;Angela M. Proell;C. Ras;W. V. van Winden;W. V. van Gulik;J. Heijnen
通讯作者：
Liang Wu;J. V. van Dam;D. Schipper;M. Kresnowati;Angela M. Proell;C. Ras;W. V. van Winden;W. V. van Gulik;J. Heijnen
影响因子：
2.6
作者：
Aboka, Fredrick O.;van Winden, Wouter A.;Heijnen, Joseph J.
通讯作者：
Heijnen, Joseph J.
影响因子：
2.7
作者：
Guan Wang;Wenjun Tang;Jianye Xia;J. Chu;H. Noorman;W. M. Gulik
通讯作者：
Guan Wang;Wenjun Tang;Jianye Xia;J. Chu;H. Noorman;W. M. Gulik
影响因子：
4.6
作者：
Liu P;Huang M;Guo M;Qian J;Lin W;Chu J;Zhuang Y;Zhang S
通讯作者：
Zhang S