Role of anchor residues in peptide binding to three HLA-A26 molecules.
Role of anchor residues in peptide binding to three HLA-A26 molecules.
复制标题
锚定残基在肽与三个 HLA-A26 分子结合中的作用。
作者：
N. Yamada;Y. Ishikawa;T. Dumrese;K. Tokunaga;T. Juji;T. Nagatani;K. Miwa;H. Rammensee;M. Takiguchi
为了研究锚残基在HLA-A26结合肽中的作用，我们使用HLA I类稳定化测定分析了各种肽与三种HLA-A26分子的结合。在20个在P2和P9处携带锚的九聚体肽中，分别有3、6和3个肽与HLA-A*2601、HLA-A*2602和HLA-A*2603结合。使用该肽在P1、P2或P9处的突变体的分析表明，P1处的酸性氨基酸和P2处的五个疏水残基（瓦尔、Thr、Ile、Leu和Phe）是三种HLA-A26分子的锚残基，而除了带正电荷的氨基酸之外，广泛的氨基酸起P9锚残基的作用。使用在P1、P2和P9处携带锚残基的38个九聚体肽进一步评价这些锚。这些肽中有19种与至少一种HLA-A26分子结合。携带所有三个锚残基的肽的HLA-A26结合肽的频率高于仅携带P2和P9锚残基的肽。这些结果表明，除了P2和P9锚外，P1锚在肽与三种HLA-A26分子的结合中起重要作用。
To investigate the role of anchor residues in HLA-A26 binding peptides, we analyzed the binding of various peptides to three HLA-A26 molecules using the HLA class I stabilization assay. Of twenty nonamer peptides carrying anchors at P2 and P9, 3, 6 and 3 peptides bound to HLA-A*2601, HLA-A*2602 and HLA-A*2603, respectively The peptide EV-IPMFSAL bound most strongly to these three HLA-A26 molecules. Analysis using mutants of this peptide at P1, P2 or P9 showed that acidic amino acids at P1 and five hydrophobic residues (Val, Thr, Ile, Leu and Phe) at P2 are anchor residues for the three HLA-A26 molecules while with exception of positively charged amino acids, a broad range of amino acids function as P9 anchor residues. These anchors were further evaluated using 38 nonamer peptides carrying anchor residues at P1, P2 and P9. Nineteen of these peptides bound to at least one HLA-A26 molecule. The frequency of HLA-A26 binding peptides was higher for peptides carrying all three anchor residues than for peptides carrying only P2 and P9 anchor residues. These results indicate that in addition to P2 and P9 anchors, the P1 anchor plays an important role in peptide binding to three HLA-A26 molecules.
HLA-B*3501-肽相互作用：肽锚定残基在与 HLA-B*3501 分子结合中的作用。
影响因子：
4.4
作者：
Takamiya,Y;Schönbach,C;Nokihara,K;Yamaguchi,M;Ferrone,S;Kano,K;Egawa,K;Takiguchi,M
通讯作者：
Takiguchi,M
与 HLA I 类 α 3 结构域反应的单克隆抗体识别的单态决定簇的结构和功能分析。
DOI：
--
发表时间：
1992
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Tanabe,M;Sekimata,M;Ferrone,S;Takiguchi,M
通讯作者：
Takiguchi,M