Suppression of Alternaria brassicicola infection by volatile compounds from spent mushroom substrates
Suppression of Alternaria brassicicola infection by volatile compounds from spent mushroom substrates
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DOI：
10.1016/j.jbiosc.2021.03.003
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发表时间：
2021-06-16
影响因子：
2.8
通讯作者：
Ishihara, Atsushi
中科院分区：
文献类型：
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作者：
Fujita, Rina;Yokono, Mizuki;Ishihara, Atsushi
大多数商业上流通的蘑菇是通过使用人工生产的蘑菇基质来培养的。然而，在蘑菇收获后，由于需要处理场地和所涉及的成本，废弃蘑菇基质（SMSs）的处理对蘑菇产业来说是一个严重的问题。因此，考虑到SMSs作为土壤改良剂可循环利用的可能性，我们研究了SMSs混合土壤对白菜叶斑病病原菌——油菜互花菌侵染拟南芥叶片的影响。在栽培土壤中混用沼地菌、小孢子菌、腐烂Lyophyllum decdecum和多毛木耳（auricaria polytricha）的SMSs，抑制了芸苔菌病害的形成。在含SMSs的土壤中培养拟南芥幼苗，其防御反应未被诱导。SMSs释放的挥发物与土壤孵育7 d后，幼苗的病变形成受到抑制。SMSs分别用于麻球菌、小孢子菌、腐烂菌、多毛菌、香菇和茶树。SMSs的挥发物降低了芸苔菌菌丝的伸长。GC-MS分析了含有土壤的SMS提取物，检测到各种挥发性化合物；其中，臭臭素、2,4-二叔丁基酚、y-十二内酯、丁酸、愈创木酚、6-戊基-2-吡咯酮和1-辛烯-3-醇对芸苔菌的抑制活性研究发现，它们抑制菌丝伸长。这些结果表明，SMSs释放的抗真菌挥发性化合物抑制了芸苔菌的感染。(C) 2021，生物技术学会，日本。版权所有。
Most commercially circulating mushrooms are produced via cultivation using artificially produced mushroom substrates. However, after mushroom harvesting, the disposal of spent mushroom substrates (SMSs) is a serious problem for the mushroom industry owing to the need for a disposal site and the cost involved. Thus, in view of the possibility of recycling SMSs as a soil modifier, we examined the effect of soil mixed with SMSs on the infection of Arabidopsis leaves by Alternaria brassicicola, the causal agent of cabbage leaf spot. The mixing of SMSs used for Hypsizygus marmoreus, Pholiota microspora, Lyophyllum decastes, and Auricularia polytricha into culture soil suppressed the lesion formation caused by A. brassicicola. The defense responses of Arabidopsis were not induced by the culturing of these seedlings in soils containing SMSs. Suppressed lesion formation was observed after the seedlings were treated with volatiles emitted from SMSs that were incubated with soil for 7 days and used for H. marmoreus, P. microspora, L decastes, A. polytricha, Lentinula edodes, and Cyclocybe cylindracea. The volatiles from the SMSs reduced the elongation of A. brassicicola hyphae. GC-MS analyses of extracts from the SMS containing soils led to the detection of various volatile compounds; among these, skatole, 2,4-di-tert-butylphenol, y-dodecalactone, butyric acid, guaiacol, 6-amyl-2-pyrone, and 1-octen-3-ol were examined for inhibitory activity on A. brassicicola and found to suppress hyphae elongation. These findings indicate that the antifungal volatile compounds emitted by the SMSs suppress A. brassicicola infection. (C) 2021, The Society for Biotechnology, Japan. All rights reserved.