Dual activation of a sex pheromone-dependent ion channel from insect olfactory dendrites by protein kinase C activators and cyclic GMP.
Dual activation of a sex pheromone-dependent ion channel from insect olfactory dendrites by protein kinase C activators and cyclic GMP.
复制标题
蛋白激酶 C 激活剂和环 GMP 对昆虫嗅树突性信息素依赖性离子通道的双重激活。
DOI：
10.1073/pnas.88.19.8520
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发表时间：
1991
影响因子：
11.1
通讯作者：
Physiologisches
中科院分区：
文献类型：
--
作者：
Frank Zufall;Hanns Hatt;Physiologisches
嗅觉传导被认为发生在昆虫嗅觉受体神经元的外树突膜上。在这里，我们表明，外部树突状细胞膜的家蚕嗅觉受体神经元似乎是专门配备了一个特定的离子通道激活低浓度的物种特异性性信息素成分。这种所谓的AC 1通道具有56 pS的电导率，并且对阳离子是非选择性渗透的。AC 1通道可被蛋白激酶C激活剂（如甘油二酯和佛波酯）和cGMP从细胞内激活，但不能被Ca 2+、肌醇1，4，5-三磷酸或cAMP激活。我们的研究结果表明，蛋白激酶C对该离子通道的磷酸化可能是性信息素开放通道的关键步骤。
Olfactory transduction is thought to take place in the outer dendritic membrane of insect olfactory receptor neurons. Here we show that the outer dendritic plasma membrane of silkmoth olfactory receptor neurons seems to be exclusively equipped with a specific ion channel activated by low concentrations of the species-specific sex pheromone component. This so-called AC1 channel has a conductance of 56 pS and is nonselectively permeable to cations. The AC1 channel can be activated from the intracellular side by protein kinase C activators such as diacylglycerol and phorbolester and by cGMP but not by Ca2+, inositol 1,4,5-triphosphate, or cAMP. Our results imply that phosphorylation of this ion channel by protein kinase C could be the crucial step in channel opening by sex pheromones.