CD166/ALCAM expression is characteristic of tumorigenicity and invasive and migratory activities of pancreatic cancer cells.
CD166/ALCAM expression is characteristic of tumorigenicity and invasive and migratory activities of pancreatic cancer cells.
复制标题
DOI：
10.1371/journal.pone.0107247
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Tanaka M
中科院分区：
文献类型：
--
作者：
Fujiwara K;Ohuchida K;Sada M;Horioka K;Ulrich CD 3rd;Shindo K;Ohtsuka T;Takahata S;Mizumoto K;Oda Y;Tanaka M
CD 166，也称为活化白细胞粘附分子（ALCAM），由包括癌症在内的多种组织中的各种细胞表达。然而，CD 166在恶性肿瘤中的作用是有争议的，特别是在胰腺癌中。本研究旨在探讨CD 166在胰腺癌中的表达及其意义。我们采用免疫组化和流式细胞术分析CD 166在手术胰腺组织和胰腺癌细胞系中的表达。通过侵袭和迁移测定以及在小鼠异种移植模型中分析分离的CD 166+和CD 166-胰腺癌细胞之间的差异。我们还进行了定量RT-PCR和微阵列分析，以评估在培养的细胞中的CD 166和相关基因的表达水平。免疫组化显示胰腺癌组织中CD 166的高表达（12.2%; 12/98）与正常胰腺对照组（0%; 0/17）相比（p = 0.0435）。 流式细胞仪检测结果显示，CD 166在胰腺癌细胞系中的表达率为0-99.5%，在胰腺癌组织中的表达率为33.8%-70.2%。CD 166-胰腺癌细胞的侵袭和迁移能力明显强于CD 166+胰腺癌细胞（p<0.05）。另一方面，CD 166 + Panc-1细胞显示出比CD 166- Panc-1细胞显著更强的集落形成活性（p<0.05）。体内分析显示，在皮下和原位小鼠肿瘤模型中，CD 166+细胞引起的肿瘤生长显著大于CD 166-细胞（p<0.05）。上皮-间质转化激活剂Zeb 1的mRNA表达在CD 166-细胞中过表达（p<0.001）。微阵列分析显示TSPAN 8和BST 2在CD 166+细胞中过表达，而BMP 7和Col 6A 1在CD 166-细胞中过表达。CD 166+胰腺癌细胞具有较强的致瘤性，而CD 166-胰腺癌细胞具有相对较强的侵袭和迁移活性。这些结果表明，CD 166表达与胰腺癌细胞的不同功能有关。
CD166, also known as activated leukocyte cell adhesion molecule (ALCAM), is expressed by various cells in several tissues including cancer. However, the role of CD166 in malignant tumors is controversial, especially in pancreatic cancer. This study aimed to clarify the role and significance of CD166 expression in pancreatic cancer. We performed immunohistochemistry and flow cytometry to analyze the expression of CD166 in surgical pancreatic tissues and pancreatic cancer cell lines. The differences between isolated CD166+ and CD166- pancreatic cancer cells were analyzed by invasion and migration assays, and in mouse xenograft models. We also performed quantitative RT-PCR and microarray analyses to evaluate the expression levels of CD166 and related genes in cultured cells. Immunohistochemistry revealed high expression of CD166 in pancreatic cancer tissues (12.2%; 12/98) compared with that in normal pancreas controls (0%; 0/17) (p = 0.0435). Flow cytometry indicated that CD166 was expressed in 33.8–70.2% of cells in surgical pancreatic tissues and 0–99.5% of pancreatic cancer cell lines. Invasion and migration assays demonstrated that CD166- pancreatic cancer cells showed stronger invasive and migratory activities than those of CD166+ cancer cells (p<0.05). On the other hand, CD166+ Panc-1 cells showed a significantly stronger colony formation activity than that of CD166- Panc-1 cells (p<0.05). In vivo analysis revealed that CD166+ cells elicited significantly greater tumor growth than that of CD166- cells (p<0.05) in both subcutaneous and orthotopic mouse tumor models. mRNA expression of the epithelial-mesenchymal transition activator Zeb1 was over-expressed in CD166- cells (p<0.001). Microarray analysis showed that TSPAN8 and BST2 were over-expressed in CD166+ cells, while BMP7 and Col6A1 were over-expressed in CD166- cells. CD166+ pancreatic cancer cells are strongly tumorigenic, while CD166- pancreatic cancer cells exhibit comparatively stronger invasive and migratory activities. These findings suggest that CD166 expression is related to different functions in pancreatic cancer cells.
登录
查看更多内容
影响因子：
3.1
作者：
Liu, Jun;Ben, Qi-Wen;Yuan, Yao-Zong
通讯作者：
Yuan, Yao-Zong
DOI：
10.1073/pnas.0703478104
发表时间：
2007-06-12
影响因子：
11.1
作者：
Dalerba, Piero;Dylla, Scott J.;Clarke, Michael F.
通讯作者：
Clarke, Michael F.
影响因子：
11.2
作者：
Li, Chenwei;Heidt, David G.;Simeone, Diane M.
通讯作者：
Simeone, Diane M.
影响因子：
4
作者：
Gu, Gongping;Zhao, Dejian;Yin, Ziming;Liu, Ping
通讯作者：
Liu, Ping
影响因子：
29.4
作者：
Bachem, MG;Sch端nemann, M;Adler, G
通讯作者：
Adler, G