Accurate identification of single-nucleotide variants in whole-genome-amplified single cells.
Accurate identification of single-nucleotide variants in whole-genome-amplified single cells.
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DOI：
10.1038/nmeth.4227
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发表时间：
2017-05
期刊：
影响因子：
48
通讯作者：
Vijg J
中科院分区：
文献类型：
--
作者：
Dong X;Zhang L;Milholland B;Lee M;Maslov AY;Wang T;Vijg J
全基因组范围内，单细胞DNA突变分析容易出现与细胞裂解和全基因组扩增相关的伪影。在这里，我们通过开发单细胞多位移扩增（SCMDA）和单细胞变异调用者（SCcaller）来解决这些问题。通过比较scmda扩增的单细胞与来自同一群体的未扩增的克隆，该方法为单细胞基因组学中体细胞突变分析的标准化提供了坚实的基础。
Genome-wide, DNA mutation analysis in single cells is prone to artifacts associated with cell lysis and whole genome amplification. Here we addressed these issues by developing Single-Cell Multiple Displacement Amplification (SCMDA) and the single-cell variant caller, SCcaller. Validated by comparing SCMDA-amplified single cells with unamplified clones from the same population, the procedure provides a firm foundation for standardizing somatic mutation analysis in single-cell genomics.