Quantitative 3D structural analysis of the cellular microstructure of sea urchin spines (II): Large-volume structural analysis
Quantitative 3D structural analysis of the cellular microstructure of sea urchin spines (II): Large-volume structural analysis
复制标题
DOI：
10.1016/j.actbio.2020.03.006
复制
发表时间：
2020-04-15
影响因子：
9.7
通讯作者：
Li, Ling
中科院分区：
文献类型：
--
作者：
Chen, Hongshun;Yang, Ting;Li, Ling
生物细胞材料一直是轻量化工程结构设计的宝贵灵感来源。在这一过程中，需要在3D中对从单个分支和节点级别到全球网络级别的生物细胞材料进行定量了解。在这里，我们采用本系列第一篇论文中演示的多尺度细胞网络分析工作流程来分析大体积(约0.32 mm(3))海胆刺的生物矿化多孔结构。利用一组全面的结构描述符来定量描述从脊柱中心到边缘区域的远程微结构变化。我们的分析表明，分支从脊柱中心到边缘逐渐延长(类似于50%增加)和粗大(类似于100%增加)，这决定了相对密度的空间变化(从类似于12%到类似于40%)。分支形态和网络组织模式也随着其位置和方向的不同而逐渐变化。此外，对单个隔膜的细胞网络的分析提供了相邻隔膜之间的互连特征，这是用于构建边缘区域细胞结构的主要结构基元。最后，结合提取的长程细胞网络和有限元模拟，我们可以有效地检查局部有效杨氏模数在脊柱半径上的空间和方向相关性。本文的结构-力学分析揭示了多孔刺的结构设计，这可能为设计和建模轻质但坚固和具有损伤容忍性的细胞材料提供重要的见解。这限制了我们对海胆脊椎三维微结构变化和机械特性之间的相互作用的理解，从而限制了生物灵感设计的基本原理的推导。这项工作首次利用我们的多尺度3D网络分析来量化3D细胞网络及其在从单个分支和节点级别到细胞网络级别的海胆脊椎中的大容量变化。本文所展示的网络分析有望对生物矿化、功能生物材料和仿生材料设计领域产生极大的兴趣。由Elsevier Ltd代表Acta Materialia Inc.出版。
Biological cellular materials have been a valuable source of inspiration for the design of lightweight engineering structures. In this process, a quantitative understanding of the biological cellular materials from the individual branch and node level to the global network level in 3D is required. Here we adopt a multiscale cellular network analysis workflow demonstrated in the first paper of this work series to analyze the biomineralized porous structure of sea urchin spines from the species Heterocentrotus mamillatus over a large volume (ca. 0.32mm(3)). A comprehensive set of structural descriptors is utilized to quantitatively delineate the long-range microstructural variation from the spine center to the edge region. Our analysis shows that the branches gradually elongate (similar to 50% increase) and thicken (similar to 100% increase) from the spine center to edge, which dictates the spatial variation of relative density (from similar to 12% to similar to 40%). The branch morphology and network organization patterns also vary gradually with their positions and orientations. Additionally, the analysis of the cellular network of individual septa provides the interconnection characteristics between adjacent septa, which are the primary structural motifs used for the construction of the cellular structure in the edge region. Lastly, combining the extracted long-range cellular network and finite element simulations allows us to efficiently examine the spatial and orientational dependence of local effective Young's modulus across the spine's radius. The structural-mechanical analysis here sheds light on the structural designs of H. mamillatus' porous spines, which could provide important insights for the design and modeling of lightweight yet strong and damage-tolerant cellular materials.Statement of SignificancePrevious investigations on the cellular structures of sea urchin spines have been mainly based on 2D measurements or 3D quantification of small volumes with limited structural parameters. This limits our understanding of the interplay between the 3D microstructural variations and the mechanical properties in sea urchin spines, which hence constrains the derivation of the underlying principles for bio-inspired designs. This work utilizes our multiscale 3D network analysis, for the first time, to quantify the 3D cellular network and its variation across large volumes in sea urchin spines from individual branch and node level to the cellular network level. The network analysis demonstrated here is expected to be of great interest to the fields of biomineralization, functional biological materials, and bio-inspired material design. Published by Elsevier Ltd on behalf of Acta Materialia Inc.