Self-Assembled Supramolecular Nanoprobes for Ratiometric Fluorescence Measurement of Intracellular pH Values
Self-Assembled Supramolecular Nanoprobes for Ratiometric Fluorescence Measurement of Intracellular pH Values
复制标题
用于细胞内 pH 值比率荧光测量的自组装超分子纳米探针
DOI：
10.1021/ac504458r
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发表时间：
2015-02-17
影响因子：
7.4
通讯作者：
Yang, Meng
中科院分区：
文献类型：
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作者：
He, Leiliang;Yang, Xiaohai;Yang, Meng
小结构单元自组装成功能性超分子纳米结构为新型材料的设计开辟了前景。利用这种分子工程策略，我们开发了自组装超分子纳米探针（SSNPs），用于细胞中pH值的比率荧光测量。通过将pH敏感的金刚烷荧光素（Ad-F）和pH不敏感的金刚烷罗丹明B（Ad-R）与β-环糊精聚合物（poly-beta-CD）混合，可制备直径约为30 nm的纳米探针。具有良好生物相容性的纳米探针已成功地应用于测量细胞内pH值在48的范围内，并估计与不同的刺激在细胞内的pH值波动。此外，我们预计这种自组装方法适用于构建细胞中其他靶点的纳米探针，只需将相应的指示染料替换为相关指示剂。
Self-assembly of small building blocks into functional supramolecular nanostructure has opened prospects for the design of novel materials. With this molecular engineering strategy, we have developed self-assembled supramolecular nanoprobes (SSNPs) for ratiometric fluorescence measurement of pH values in cells. The nanoprobes with a diameter of similar to 30 nm could be formulated just by mixing pH-sensitive adamantanefluorescein (Ad-F) and pH-insensitive adamantaneRhodamine B (Ad-R) with beta-cyclodextrin polymer (poly-beta-CD) at one time. The nanoprobes with good biocompatibility have been successfully applied to measure intracellular pH in the pH range of 48 and estimate pH fluctuations associated with different stimuli in cells. Moreover, we expect that this self-assembled approach is applicable to the construction of nanoprobes for other targets in cells just by replacing the respective indicator dyes with relevant indicators.